Episc esc episc growth media what factors are

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EpiSC ESC EpiSC growth media What factors are responsible for this early stage of chromatin reconfiguration? Explore this transition in vitro with ESCs carrying mutations or deletions of chromatin modifying components
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Diversity in chromatin architecture Mouse Fibroblast (MEF) Embryonic Stem (ES) Cell Primary Mouse Lymphocyte What changes to the chromatin landscape accompany somatic cell reprogramming? 500 nm
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MEF iPS cell Dispersion of chromocentre heterochromatin in iPSCs Correlative Fluorescence Microscopy/ESI Chromatin yellow Protein-based structures blue 500 nm
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Disruption of constitutive heterochromatin is a hallmark of full reprogrammed iPS cells and pluripotent cells Full iPS cells Partial iPS Cell Parental MEF Ugljesa Djuric, Eden Fussner
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Heterochromatin reorganization of partial iPS cells by Mek/Gsk3 2i inhibitor treatment 2i- based dispersal of constitutive heterochromatin is accompanied by retroviral silencing of transgenes late stage event in re-programming H3K9Me3 domain H3K9Me3 domain Eden Fussner, Ugi Djuric
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EOS3F-29 EOS3F-24 Parental MEF Fully reprogrammed Partially reprogrammed Only 10 nm chromatin fibres observed in dispersed CCs in reprogrammed cells Do 30 nm chromatin fibres unfold to 10 nm fibres upon reprogramming, assuming there were 30 nm fibres to begin with.
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Chromocentres and surrounding RNPs visualized with ESI 0.2 μ m Eden Fussner
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Chromocentres and surrounding RNPs visualized with ESI 0.2 μ m
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Model: Hierarchy of fibre types required to compact chromatin Olins et al. Science 1974 Horowitz et al. JCB 1994 Horn and Peterson Science 2002
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