7 place your spreader in the microtiter pippete tips

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7. Place your Spreader in the “Microtiter Pippete Tips” Waste Beaker. 8. Tape your Nutrient Agar Plates together in two Stacks of 3 Plates each (or one Stack of 6) and incubate them at 30°C for 48 Hours . (2) MacConkey Agar Plate Spread Plating S 1. Spread Wallah places the “8” MacConkey Agar Plate in the Center of your Turntable, Bottom Side Down/Lid Side Up. V 2. Vortexer vortexes the 10 -7 Dilution Tube and Pipetter uses a new Pipette Tip P to transfer 100 μ l onto the Surface of this “8” MacConkey Agar Plate . Pipetter : Remember to use a new Pipette Tip for your MacConkey Agar Plates. S 3. Spread Wallah removes a new sterile Spreader from the Packet. S 4. Spread Wallah slowly spins the Turntable, lifts the Lid and uses their Spreader to evenly spread this Sample across the Surface of this Plate. S 5. Spread Wallah replaces the Lid and moves the Plate somewhere else. 6. Repeat Steps 1-5, using the appropriate Dilution Tube for your 7, 6, 5, 4 and 3 Nutrient Agar Plates . 7. Place your Spreader in the “Microtiter Pippete Tips” Waste Beaker. 8. Tape your MacConkey Agar Plates together in two Stacks of 3 Plates each (or one Stack of 6) and incubate them at 37°C for 24 Hours .
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Lab 6 Page 9 Water Quality Testing Materials (per Clan): • 1 Sterile 250 ml Flask • 3 Tubes of Double Strength LSL Broth (Governator Tubes with red Caps) • 6 Tubes of LSL Broth (Blue Caps) • Disposable 5 ml Serological Pipette (and Lightsaber) • P1000 Microtiter Pipette (and Tips) • P200 Microtiter Pipette (and Tips) • A Plastic Container Procedure 1. Label 3 Pieces of Tape with the Roman Numeral “M” (“1000”). • Put one of each of these Labels on 3 of your Blue Cap LSL Broth Tubes 2. Label 3 Pieces of Tape with the Roman Numeral “C” (“100”). • Put one of each of these Labels on the remaining 3 of your Blue Cap LSL Broth Tubes 3. Aliquot ~50 ml of our Potty Water Sample from the Liter Flask on the Back Bench into your Sterile 250 ml Flask. This Water contains E. coli . It ʼ s a Biohazard. 4. Use a Serological Pipette to add 10 ml of your Water Sample to each of your 3 large red Cap Tubes of Double Strength LSL Broth. • Gently Finger Vortex each Tube until the Water Sample and the Double Strength LSL Broth are thoroughly mixed and you can no longer see Schlieren (German= streaks) , the Lines produced by sharp Boundaries between the Water and the LSL Broth, which have different Refractive Indices. 5. Use a large Pipette Tip and your P1000 Microtiter Pipette to add 1000 μ l (1 ml) of your Water Sample to each of your 3 “1000” Tubes of LSL Broth. • Set your P1000 Microtiter Pipette at 1 | 0 | 0 for 1000 μ l. - Gently Finger Vortex each Tube. Schlieren will be less Visible in these Tubes. __________________________________________ I suggest you give me what you owe me... or 'Anything Goes’!
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