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06.The Count

V 2 vortexer vortexes the 10 7 dilution tube and

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V 2. Vortexer vortexes the 10 -7 Dilution Tube and Pipetter transfers 100 μ l P (P200 at 1 | 0 | 0) onto the Surface of this “8” Nutrient Agar Plate . S 3. Spread Wallah removes a sterile Spreader from the Packet. S 4. Spread Wallah slowly spins the Turntable, lifts the Lid and uses their Spreader to evenly spread this Sample across the Surface of this Plate. Spread your Sample thoroughly, but expose the Surface of your Plate to the Air for as short a Time as possible. S 5. Spread Wallah replaces the Lid and moves the Plate somewhere else. 6. Repeat Steps 1-5, using the appropriate Dilution Tube for your 7, 6, 5, 4 and 3 Nutrient Agar Plates . 7. Place your Spreader in the “Microtiter Pippete Tips” Waste Beaker. 8. Tape your Nutrient Agar Plates together in two Stacks of 3 Plates each (or one Stack of 6) and incubate them at 30°C for 48 Hours . (2) MacConkey Agar Plate Spread Plating S 1. Spread Wallah places the “8” MacConkey Agar Plate in the Center of your Turntable, Bottom Side Down/Lid Side Up. V 2. Vortexer vortexes the 10 -7 Dilution Tube and Pipetter uses a new Pipette Tip P to transfer 100 μ l onto the Surface of this “8” MacConkey Agar Plate . Pipetter : Remember to use a new Pipette Tip for your MacConkey Agar Plates. S 3. Spread Wallah removes a new sterile Spreader from the Packet. S 4. Spread Wallah slowly spins the Turntable, lifts the Lid and uses their Spreader to evenly spread this Sample across the Surface of this Plate. S 5. Spread Wallah replaces the Lid and moves the Plate somewhere else. 6. Repeat Steps 1-5, using the appropriate Dilution Tube for your 7, 6, 5, 4 and 3 Nutrient Agar Plates . 7. Place your Spreader in the “Microtiter Pippete Tips” Waste Beaker. 8. Tape your MacConkey Agar Plates together in two Stacks of 3 Plates each (or one Stack of 6) and incubate them at 37°C for 24 Hours .
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Lab 6 Page 9 Water Quality Testing Materials (per Clan): • 1 Sterile 250 ml Flask • 3 Tubes of Double Strength LSL Broth (Governator Tubes with red Caps) • 6 Tubes of LSL Broth (Blue Caps) • Disposable 5 ml Serological Pipette (and Lightsaber) • P1000 Microtiter Pipette (and Tips) • P200 Microtiter Pipette (and Tips) • A Plastic Container Procedure 1. Label 3 Pieces of Tape with the Roman Numeral “M” (“1000”). • Put one of each of these Labels on 3 of your Blue Cap LSL Broth Tubes 2. Label 3 Pieces of Tape with the Roman Numeral “C” (“100”). • Put one of each of these Labels on the remaining 3 of your Blue Cap LSL Broth Tubes 3. Aliquot ~50 ml of our Potty Water Sample from the Liter Flask on the Back Bench into your Sterile 250 ml Flask. This Water contains E. coli . It ʼ s a Biohazard. 4.
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V 2 Vortexer vortexes the 10 7 Dilution Tube and Pipetter...

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