At first i thought of setting it at a yard and a half

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We must fix the daily work quota for your men. At first I thought of setting it at a yard and a half, so as not to overtire them, but don't you think it would be best if we make it the same as the British soldiers? That would also create a healthy competitive spirit.” I have already given the order.’ © 1957 Horizon Pictures
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Lab 7 Page 11 Gram Negative Biochemical Tests F Agar Check your F Agar Plates and our F Agar Control Plate • Positive = Green-to-Yellow Fluorescence (Pseudomonas ) • Negative = No Fluorescence (E. coli or Enterobacter ) IMViC Indole (Atlas Page 74) Check your Indole Tubes (Tryptone Broth) 1. Vortex your Indole Tube (Low Speed) 2. Add 4 Drops Kovac’s Reagent (in the Ice Bath) 3. The Reaction should occur within a few Seconds • Positive = Magenta Ring forms at the Top of the Tube (E. coli ) • Negative = No Red Ring forms at the Top of the Tube (Enterobacter ) MR-VP Broth Tubes (Methyl Red / Voges-Proskauer) 1. Vortex each of your MR-VP Tubes (which have Stainless Steel Caps) 2. Aliquot Half of the Contents of each Tube into a new, labeled Test Tube (one with a White Plastic Cap) 3. Use the Original Tube (Stainless Steel Cap) for the Methyl Red Test 4. Use the New Tube (White Plastic Cap) for the Voges-Proskauer Test ___________________________________________________________ “One day the war will be over. And I hope that the people that use this bridge in years to come will remember how it was built and who built it. Not a gang of slaves, but soldiers, British soldiers, Clipton, even in captivity. ‘The fact is, what we're doing could be construed as - forgive me sir - collaboration with the enemy. Perhaps even as treasonable activity. Must we work so well? Must we build them a better bridge than they could have built for themselves?’ “You're a fine doctor, Clipton, but you've a lot to learn about the army.” © 1957 Horizon Pictures
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Lab 7 Page 12 Methyl Red (Atlas Page 82) 1. Add 4 Drops of Methyl Red (on the Back Lab Bench) to the Tubes with the Stainless Steel Caps. 2. Vortex (Low Speed) 3. The Reaction should occur within a Minute or so • Positive = Red (but probably Jolly Rancher Watermelon) (E. coli ) • Negative = Yellow (but probably Cantaloupe or Dried Mango) (Enterobacter ) Voges-Proskauer (Atlas Page 98) 1. Add 12 Drops α -Naphthtol (in the Ice Bath on the Back Bench) to the Tubes with the White Plastic Caps 2. Vortex (Low Speed) Your Tube will look like Yoo Hoo Chocolate Drink. 3. Add 4 Drops KOH (in the Ice Bath) KOH is clear. So is H 2 O 2 . Check the Label. 4. Vortex (Low Speed) 5. The Reaction may take as long as 45 Minutes • Positive = Red (in the Upper Half of the Tube) (Enterobacter ) • Negative = No Change (or Copper-color) (E. coli ) Citrate (Atlas Page 64) Check your Citrate Slants • Positive = Offshore Open Ocean Malibu Blue (Enterobacter ) • Negative = Cool and Refreshing Coastal Redwoods Green (E. coli )
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Lab 7 Page 13 Biochemical Practical The Unknown Bacteria are the same eight Bacteria from our Rogues Gallery (and some were Controls for the Biochemical Tests).
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