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Lucci_Prelab11_Monday2pm

13 how are the wells in agarose gel formed they are

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13. How are the wells in agarose gel formed? They are preformed by the casting trays that the gel is poured into after mixing the dry polymer in boiling buffer and set to cool at room temperature. 14. Define the following: Cathode – negative electrode (supplies electrons to conductive buffer solution) Anode – positive electrode 15. At neutral pH, what is the charge of DNA (or RNA) and in what direction (use the above terms describing the electrodes) does in travel in an electric field? Negatively charged because of the negative charges on the phosphate backbone. They travel from the cathode (-) toward the anode (+). 16. What is the electrophoresis buffer? 0.4M Tris-Acetate-EDTA (TAE), pH 8.0 17. In this lab you will be using SYBR-Green as the nucleic acid stain. This stain works the same way ethidium bromide does. What is the purpose of the stain? Nucleic acids are not colored and therefore need to be stained so that they are visible & their position could be acquired during electrophoresis. 18. The sample buffer contains glycerol and bromphenol blue. What is the purpose of each of these? The viscous glycerol ensures that the samples will layer smoothly at the bottom of
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the sample wells. Bromphenol blue works as a tracking dye so the observer can follow the progress of the electrophoretic run. 19. What precaution should you take when micropipetting the DNA or dye samples into the wells? NOT to punch the tip of the pipette through the bottom of the gel. Exercise 11. 1 20. What is the purpose of this exercise? Introduce plasmid pUC18 into E. coli in order to create a population of bacteria cells resistant to the antibiotic ampicillin. 21.
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13 How are the wells in agarose gel formed They are...

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