Anaerobic endospore formers clostridium tetani

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Anaerobic Endospore Formers - Clostridium tetani. (Tetanus, Botulism) Clostridium tetani causes Tetanus. Many Wounds -- particularly in a War Zone -- come into Contact with Soil containing Clostridium tetani Spores and most Wounds become Anaerobic, providing the Ideal Environment for this Bacterium. Tetanus is extremely Rare in the US but fairly common in Developing Tropical Countries. - Clostridium botulinum Clostridium botulinum causes Botulism. The Inside of a Can is Anaerobic, which is Ideal for the Growth of this Bacterium. A Can doesn ʼ t necessarily have to be bulging to contain Clostridium botulinum . - Clostridium perfringes Clostridium perfringes causes Gas Gangreen. When grown inside a Human, it has a Doubling Time of 8 to 10 Minutes. It can also cause Food Poisoning. - Clostridium difficile Clostridium difficile is a Normal Component of the Human Gasterointestinal Tract Microflora. It is more Resistant to Antibiotics than most over Intestinal Microflora and so Treatment with Antibiotics can result in Clostridium difficlie Infection and Colitis.
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Growth of Bacteria Page 4 Spore Staining We used the Heat Resistance of Endospores (Spores) to select for our Bacillus Isolates. Bacterial Spores are also resistant to many Chemicals, including some Disinfectants and Stains (the unstained Regions on Gram Stained Bacillus are the Spores). Our Spore Staining Procedure involves making a Smear of our Bacillus cereus , heat-fixing it, and then flooding the Smear with Malachite Green and using Heat to drive this Stain into the Spores (and the Vegetative Portion of the Cells). It ʼ s relatively easy to destain the Vegetative (“Non-Spore”) Portions with Water, but the Malachite Green will stubbornly remain in the Spores. We ʼ ll counterstain the Vegetative Portion of the Cell with Safranin. The End Result (Atlas Figure 5-22, Page 41) will be Red Bacteria with Green Endospores, which lends a Festive Touch to this particular Exercise.
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