07.Epidemic_Biochemicals

# At this point 2 students should have jawa fever 3 at

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Lab 7 Page 7 Viable Counts (Atlas Page 217) 1. Look at all of your Plates but check for Plates with between 30 and 300 Colonies. Colonies can overlap and still be counted. If a Plate contains more than 300 Colonies, make a Notation of TNTC (Too Numerous To Count.). Use your Sharpie to mark Plates with a whole lot of Colonies into Quadrants, count the Number of Colonies within one Quadrant, and then multiply this Number by Four. 2. The number of Viable Bacteria per Gram of Hamburger will simply be equal to the Number of Colonies on a Plate divided by the Dilution Factor of that Plate. Example: • There are 150 Colonies on your “7” Nutrient Agar Spread Plate. • Divide 150 by the Dilution Factor: 150 ÷ 10 -7 = 150 x 10 7 = 1.5 x 10 9 • That’s 1.5 x 10 9 Colony Forming Units per Gram of Hamburger Sample 3. Remember -- • Nutrient Agar Spread Plates are used to determine the Total Number of Aerobic Bacteria per Gram of Hamburger. • MacConkey Agar Spread Plates are used to determine the Number of Enteric Bacteria per Gram of Hamburger. Your Viable Count Plates will be seriously Stinky _____________________________________________________ “You mean, you intend to uphold the letter of the law, no matter what it costs?” Without law, Commander, there is no civilization.’ That's just my point; here, there is no civilization.” Then we have the opportunity to introduce it.’ © 1957 Horizon Pictures

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Lab 7 Page 8 Water Quality Testing (MPN) 1. Within each Set of 3 Tubes, determine the Number of Tubes that are Positive (Turbid [“Cloudy”] + Gas). It’s likely that all 3 of your Red Cap Tubes are
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