Β rangtp β no fret fret cfp cfp yfp yfp kalab 2006

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β RanGTP β No FRET FRET CFP CFP YFP YFP Kalab 2006 .Nature.440:697-701 Gradient model Gradient steep enough to impart spatial information General activator model Gradient is too shallow to impart spatial information
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RanGTP is a spatial cue for microtuble organization in mitosis hypothesis: If Ran is a spatial cue, move it and the microtubules should follow RCC1 repositioned on the plasma membrane plasma membrane asters now formed wildtype RCC1 on the plasma membrane microtubules grow past chromosomes to the plasma membrane Zonis and Wilde 2011
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Kinetochores nucleate microtubules during mitosis full nocodozle washout partial nocodozole washout GFP-tubulin expressing cells centrosomes non-centr o some sites microtubules nucleated from kinetochores microtubules survivin ...kinetochore marker kinetochore nucleation dependent upon TPX2 Ran pathway survivin MCAK Tulu et al 2006 Curr. Biol. 16: 536-541
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QVT %&'+ %QTG %&'++ %&'+++ KOT KOT QVT 'WEJTQOCVKP 'WEJTQOCVKP *GVGTQEJTQOCVKP *±-²OG³´ *±-²OG±´ *µ-³¶OG± ··¸s·³¶ DR µ¶s·¶¶ DR 7R VQ ¹ /D
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