Transcriptional control evidence 1 differential

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Transcriptional Control Evidence 1) differential transcription of genes 2) nuclear run-on transcription assays Two-Stage Process 1) decondensation of coiled chromatin 2) regulated transcription of uncoiled region Binding of Transcriptional Factors Regulates Transcription Regulatory proteins Consensus binding sites Combinatorial model for gene regulation Cis-Acting Elements: Eukaryotic Promoters and Enhancers
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Promoters Upstream promoter region Enhancers Deletion mutant technique Trans-Acting Factors: Regulatory Proteins Bind to Promoters and Enhancers 2 Structural Domains 1) DNA binding domain 2) transcription activation domain 3) Common Structural Motifs 1) Helix-turn-helix 2) Zinc finger 3) Leucine zipper Mechanisms of Action of Enhancers and Transcriptional Factors Long range chromatin effect Gateway for liner diffusion Looping/interaction Possible Role of DNA Methylation in Regulating DNA Availability Methylation of cytosine DNA of inactive gene tends to have more methylation. Methylation Posttranscriptional Control RNA Processing and Translocation Alternative splicing Translational control 1) Selective utilization of specific mRNA 2) Variation in rates of mRNA degradation 3) Availability of tRNA and tRNA synthetase 4) Prosthetic group availability example: regulation of transcription by Hemin in red blood cells Posttranslational Control Permanent Modification Glycosylation Proteolytic actions Reversible structural modification phosphorylation Responses to Intracellular Elements Ca ++ , cAMP, IP 3
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  • Fall '08
  • Johnson,M
  • cell biology, DNA, DNA binding domain, transcriptional factors, transcription activation domain, Factors Regulates Transcription, nuclear run-on transcription

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