Special Stains NMCSD Flashcards

Terms Definitions
types of mucopolysac
1. neurtal: glands in GI tract, prostate, PAS +
2. Acid (simple or non-sulfated) epitheial cells cotain sialic acid PAS+, alicain blue at 2.5, colloidial iron, metachromatic, resist hyaluronidase
3. Acid (simple mesenchymal) contain hyaluronic acid tissue stroma, DO NOT STAIN WITH PAS, do stain with alcian blue at 2.5, colloidal iron, metachromatic dyes, no resit with hyaluronicacid, sarcomas
4. Acid (complex, sulfated, epithelia) adenocarcinomas, PAS +, alcian blue at 1, colloidial iorn, mucicarmine, metachromatic resist hyauronidase
5. Acid (complex, connective tissue) stroma, cartilate, bone chondrotin sulfate or kearatin PAS negative, alcian blue at 0.5
colloidal iron "AMP" requires?
formalin fixation
colloidal iron blue color
prussian blue
mucicarmine stain uses? stains epithelial mucin?
carmine (alum lake)
epithelial mucin red
cryptococcus
mucin stain with most specificity?
mucicarmine very SPECIFIC
VERY INSENSITIVE
most sensitive mucin stain?
PAS
colloidal iron predictable?
no
Mucin Stains
PAS most sensitive
Mucicarmine most specific
Alcian blue
Colloidal Iron not predicatble
Masson's Trichrome stain
nuclei deep blue
skeletal and smooth muscle red
collagen and mucin light blue
Masson Trichrome 4 dyes used?
hematoxylin
Biebrich scarlet
acid fushsin
aniline blue
Masson's Trichrome keratin and muscle fibers?
red
Masson's Trichroime collagen and bone
blue or green
Masson's Trichrome cytoplasm?
light red or pink cytoplasm
Masson's Trichrome cell nuclei?
brown to black
Weigert's iron hematoxylin?
used in Masson's Trichrome

sequence of 3 solutions
ferric chloride in diluted hydrochloric acid
hematoxylin in 95% ethanol
postassium ferricyanide solution alkalized by sodium borate

used to stain nuclei blue
Solution A in Masson's Trichrome?
plasma stain
acid fuchsin, Xylidine Ponceau, glacial acetic acid, and distilled water

stains keratin and muscle red
Solution B Masson's Trichrome?
phosphomolybdic acid in distilled water
Solution C in Masson's Trichrome?
fibre stain
light green SF yellowis or Fast Green FCF
stains collagen
i f blue then use
methyl blue, water blue, ora niline blue can be used
Verhoeff-Van Gieson Stain?
also called elastic-Van Gieson Stain (EVG)

elastic fibers blue-black to black
collagen pale red
other tissue yellow
nuclei blue to black

used to stain elatidc material vascular malfomations
only lipid stain that can be used on paraffin-embeded tissue
Osmium tetroxide
JXG stains
CD68, lysozyme, HAM56, factor 13a
xanthoma disseminatum stains
CD68, lysozyme, factor 13a
multicentric reticulohistiocytoma stains
CD68, HAM56
NXG
lysozyme, CD68, CD11b
SUPERFICIAL ACRAL FIBROMYXOMA STAINS
CD34, CD10, VIMENTIN, FACTOR 13A (scattered cells)
Mammary Paget's stains
CK7+
GCDEFP, cathepsin B, EMA

variable CEA

CK20 neg
Primary extramammary Paget's stains
S2 mammary paget's but GCDFP negative and more mucin (+mucicarmine, alcian blue 2.0, Hale's collodial iron, PAS)

CK7+ EMA+ cathepsin B +
variable DEA
Secondary extrammamary Paget's
assoc w internal malignancy
Lung carcinoma CK7+/CK20 neg
Colon CK7-, CK20+
Bladder CK7+/CK20+
Prostate CK7-/CK20-
RENAL CELL CARCINOMA STAINS
RCC-MA+
EMA+
CD10+
CAM 5.2+
CEA+
GLYCOGEN
hhf-35
PAN-MUSCLE ACTIN
SMOOTH MUSCLE STAINS
TRICHROME RED
SMA
MUSCLE SPECIFIC ACTIN
PAN-MUSCLE ACTIN: HHF-35
DESMIN
CALPONIN
H-CALDESMON
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