Zebra Fish in Research Flashcards

Animal Husbandry And Disease
Terms Definitions
Danio rerio
Zebra Fish
Adult size of zebra fish
2 inches (6 cm)
Sexual maturity of zebra fish
3 months
life span of zebra fish
5 years
Why use zebrafish in research?
- Adults are small
- Short generation time
- Large numbers of progeny
-- Fecundity (1 pair produce 1,000s per year
-- Production every 2 weeks
- Eggs externally fertilized
- Non-invasive Flourescence Imaging
-- The Daniobath
- Mutations provide isights into function
How much does a line of fish cost?
~ $2,000
What aspects of the zebrafish are used for clinical evaluation?
- Character and color of fins/scales
- Motor skills
-- Buoyancy control
-- Location in the water
- Frayed fins
- Raised Scales
- Skeletal deormities
- Abdominal distension
- Gill color
- Hemorrhaging
- General body condition
-- (anorexia, lethargy, emaciation, etc.)
Common diseases of zebrafish
- Ich
- Swim Bladder
- Gas Bubble disease
- "Skinny fish" Disease
- Tail Rot
- Ulcer
- Fungus
- Gravid
- Enlarged Heart
- Mycobacteriosis (Fish TB)
- Dropsy
Prevention of Common Diseases in Zebrafish
- Source of Fish, Water, Feed
- Reduce Stress
-- Crowding, low water flow, handling
- UV light
- Quarantine
- Cleanliness
-- Wear Gloves! Potential Zoonosis
- Disinfection of systems
Nutrition of adult zebrafish
- Brine Shrimp (Artemia salina)
- Flake Food
- Krill / Mosquito larvae
Nitrition of juvenile zebrafish
- Powder food
- Paramecium
-- unicellular ciliate microorganisms
Tank Cleaning fo zebrafish
- Bleach sterilizer
-- 1/2 cup per 10 gal
-- Let air dry overnight
-- Rinse throughly next day
- Leave algae if gentle rub does not remove
- Siphon large debris from adult tanks
Systems for zebrafish
- Life Support
- Technological Advancements
- Re-circulating Systems
-- Biological (media substrate)
-- Mechanical (remove solids)
-- Ultraviolet (disinfection)
-- Chemical (carbon - removes organic matter)
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