Parasitology Lab Exam Flashcards

Entamoeba histolytica
Terms Definitions
Dog/Cat Lungs
Paragonimus
Dog Skin
Sarcoptes
Dog/Cat Feces
Isospora
Pig Skin
Haematopinus
Dog Feces
Trichuris
Horse Skin
Stomoxys
Goat Skin
Tabanus
Ruminant Feces
Balantidium
Horse feces
Strongyloides
Horse Muscle
Sarcocystis
Pig Feces
Fasciola
Ruminant Skin
Damalinia
Pig Skin
Demodex
Ruminant Skin
Psoroptes
Dog/Cat Feces
Toxocara
Pig Feces
Metastrongylus
Dog Skin
Dermacentor
Poultry Skin
Dermanyssus
Dog/Cat Skin
Pthirus
Poultry Cecum
Eimeria
Dog/Cat Lungs
Paragonimus
Dog Liver
Leishmania
Dog Muscle
Trypanosoma
Cow Ear
Otobius
Ruminant Blood
Trypanosoma
Horse Skin
Echidnophaga
Horse feces
Eimeria
Horse Leg
Gastrophilus
Ruminant Feces
Fasciola
Ruminant rumen
Paramphistomum
Dog/Cat Small Intestine
Taenia
Horse ileocecal junction
Anoplocephala
Cat Bile/Pancreatic Duct
Platynosomum
Dog/Cat Small Intestine
Spirometra
Horse ileocecal junction
Anoplocephala
Pig Muscle
Taenia cysticercus
Dog Small Intestine
Nanophyetes
- genus species stageS
Found in unclean horse stall
Phormia
Found in unclean horse stall
Phormia
genus species stages
Composite showing various morphological forms of P. malariae. ri = ring; tr = trophozoites; sz = schizont; sg = segmenter; gm= gametocyte; p = platelets
Collected from burrow of a wild rat
Ornithodorus
genus species stage
Basket-form trophozoites of P. malariae in a thin blood smear.

In developing trophozoites of P. malariae, chromatin is rounded or streaky and the cytoplasm is usually compact with no vacuole. Pigment may be coarse and peripheral. As the trophozoites mature, the cytoplasm may elongate across the host RBC, forming a 'band-form', or may be oval with a vacuole forming a 'basket-form'. Chromatin is usually in a single mass, less definite in outline. Pigment granules become larger and tend to have a more peripheral arrangement.
name genus species stage
Plasmodium falciparum
numerous rings without mature forms
slightly smaller
marginal forms
Collected from burrow of a wild rat
Ornithodorus
List the important steps of the American Trypanosome Life Cycle
Humans: metacyclic trypomastigotes, amasitgotes in muscle (replication),
trypomastigote.
Triatomine: trypomastigote, converts to epimastigote in midgut, matures to metacyclic trypomastigote in hindgut
- genus, species, stage
-
This specimen was fixed and prepared soon after collection as evidenced by the trophozoites (marked with arrowheads) with highly extended pseudopodia which are indicative of highly motile amebas. In addition, a few precysts, containing chromatoid bodies and 1 or 2 nuclei, are present. An ameba which as started to round up and become cyst like is denoted with an asterisk (*). However, it still has a pseudopodium (arrow) which is a feature of trophozoites. Numerous polymorphonuclear cells (PMNs) are also present on this slide. These can be sometimes confused with the cysts. The nuclear morphology is a key feature which helps to distinguish PMNs from cysts.
name genus species stage and labeled parts
giardia labdia late trophozoite
Who am i?
Cysts of E. histolytica/E. dispar in a concentrated wet mount stained with iodine.
who am i and at what stage?
Entamoeba histolytica/Entamoeba dispar trophozoites have a single nucleus and usually measure 15 to 20 µm (range 10 to 60 µm), tending to be more elongated in diarrheal stool.

Trophozoites of E. histolytica/E. dispar in a direct wet mount stained with iodine.
who am i and what stage?
Trophozoites of E. histolytica. The specimen was preserved in poly-vinyl alcohol (PVA) and stained with trichrome. The vacuolated cytoplasm may be the result of less than optimal preservation. PCR was performed on this specimen to differentiate between E. histolytica and E. dispar.
genus species stage (not normal stain, but cool pic)
Giardia lamblia cyst. Chlorazol black.
CDC/Dr. George R. Healy
name genus species and stage
Trypanosoma brucei ssp. in thin blood smears stained with Wright-Giemsa.

The two Trypanosoma brucei subspecies that cause African trypanosomiasis, T. b. gambiense and T. b. rhodesiense, are indistinguishable morphologically. A typical trypomastigote has a small kinetoplast located at the posterior end, a centrally located nucleus, an undulating membrane, and a flagellum running along the undulating membrane, leaving the body at the anterior end. Trypomastigotes are the only stage found in patients. Trypanosomes range in length from 14 to 33 µm.
what is the american trypanosome life cycle
Humans: metacyclic trypomastigotes - convert to amastigote in muscle and replicates, converts to trypomastigote and released to blood
Triatomine: trypomastigote, converts to epi in midgut and replicates, goes to hindgut to mature to metacyclic trypomastigote - excreted in feces
genus, species, stages
- What am I doing?
Entamoeba histolytica troph and cyst
- I am encystating
trophozoite rounds up
secretion of cyst wall
aggregation of ribosomes (= chromatoid bodies)
2 rounds of nuclear division (14 nuclei)
list genus species and stage - what type of slide is this?
Giardia lamblia (wet mount cycst)

This organism is a pathogenic flagellate. This animated GIF shows the view one would see as they focus up and down through the organism’s cyst stage in a wet mount preparation.
name genus species stage? How do you know this?
T. cruzi in the blood exhibits typical trypomastigote morphology (see figure below). Note the lightly staining cytoplasm, spherical or slightly oval nucleus, and the large spherical kinetoplast near the posterior end. The kinetoplast sometimes appears to be larger than the diameter of the organism at its pointed end.
name genus species stage? How do you know this?
Three T. cruzi trypomastigotes in a thin blood smear stained with Giemsa.
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