bioinformatics
use of computers and mathematics to store and analyze biological data
clustered regularly interspaced short palindromic repeats (CRISPR)
group of bacterial DNA sequences that are used to defend against viruses. The system can be altered and used to target and modify specific gene sequences.
dideoxy sequencing
method used to obtain the nucleotide sequence of a strand of DNA
DNA amplification
process by which many copies of a piece of DNA are made in a laboratory
DNA cloning
use of recombinant DNA technology to replicate DNA within a rapidly reproducing organism
DNA microarray assay
use of a large number of single-stranded DNA pieces attached to a microscope slide in a grid pattern to determine gene expression
DNA primer
anchor and starting point for DNA polymerase to add free nucleotides to a growing strand of DNA in the lab
gel electrophoresis
technique that separates DNA and other molecules based on their size using electric charge
genetic engineering
direct modification of an organism's genome to change an organism's traits
green fluorescent protein (GFP)
protein common in jellyfish that glows green in the presence of ultraviolet light and is often inserted into other organisms
plasmid
small, circular piece of bacterial DNA that replicates on its own and can be transferred between cells
polymerase chain reaction (PCR)
technique for rapidly amplifying a section of DNA
recombinant DNA
DNA created from more than one individual, possibly of different species
reporter gene
gene that encodes a protein that can be easily tracked and, when attached to another gene or sequence of genes, the reporter gene will alert researchers to their expression
restriction site
place on a DNA strand that contains specific sequences of nucleotides and can be recognized by restriction enzymes
RNA interference (RNAi)
blocking of a gene's expression by a small segment of interfering RNA
vector
carrier of recombinant DNA