DNA Technology and Genomics

Vocabulary

bioinformatics

the use of computers and mathematics to store and analyze biological data

biotechnology

the use of organisms or their products for the benefit of humans

complementary base pairing

the alignment of nucleotide bases according to specific rules: adenine always pairs with thymine (or uracil in RNA), and cytosine always pairs with guanine

CRISPR

group of bacterial DNA sequences that are used to defend against viruses; can be used to target and modify specific gene sequences

DNA amplification

the process by which many copies of a piece of DNA are made

DNA cloning

use of recombinant DNA technology to replicate DNA within a rapidly reproducing organism

DNA ligase

enzyme that assists in joining DNA strands

DNA primer

anchor and starting point for DNA polymerase to add free nucleotides to a growing strand of DNA

endonuclease

enzyme that cuts DNA at a specific sequence point

gel electrophoresis

technique that separates DNA and other molecules based on their size using an electric charge

gene annotation

process of determining the function of a gene and its protein

gene therapy

use of recombinant DNA technology to modify a person's genes to combat a disease

genetic engineering

direct modification of a genome to change an organism's traits

genetically modified organism (GMO)

organism whose genome was modified through the addition of recombinant DNA

genome

the genetic material of an organism

genomics

study of an organism's entire set of DNA and how its genes interact

nuclear transfer

process of placing the nucleus of a body cell into an enucleated egg cell in order to create a clone

plasmid

small, circular piece of bacterial DNA that replicates on its own and can be transferred between cells

pluripotent

ability of a cell to give rise to many different cell types

polymerase chain reaction (PCR)

technique for rapidly producing many copies of a section of DNA

recombinant DNA

DNA created from more than one individual, possibly of a different species

repetitive DNA

non-coding regions of DNA that include repeating sequences

stem cell

cell able to reproduce indefinitely and develop into other types of specialized cells

totipotent

ability of a cell to be able to give rise to an entire organism from a single cell

transformation

uptake and incorporation of foreign DNA by a bacterial cell

transgenic

containing recombinant DNA from another species

vector

carrier of recombinant DNA