Methods and Applications of Genetic Engineering

organism used to replicate a DNA sequence

group of bacterial DNA sequences that is used to defend against viruses. The system can be altered and used to target and modify specific gene sequences.

DNA molecule synthesized from a single-stranded RNA template by a reverse transcriptase enzyme

identical copy of a DNA molecule replicated in a population of cloning hosts

enzyme in DNA replication that assembles the new strands of DNA from the template strands

any of the techniques used for differentiating between two unrelated individuals' DNA

carrier of recombinant DNA

technique that separates DNA and other molecules based on their size using an electric charge

short sequence of DNA with a radioactive or fluorescent label

use of recombinant DNA technology to modify a person's genes to combat a disease

collection of cloning hosts that collectively contain complementary DNA of all expressed genes from a cell or organism

single-stranded short RNA molecule used by CRISPR that is complementary to a DNA sequence

bind with complementary single-stranded DNA sequences

small, circular piece of bacterial DNA that replicates on its own and can be transferred between cells

technique for rapidly producing many copies of a section of DNA

DNA created from more than one individual, possibly of a different species

enzyme that cuts DNA at specific locations

small piece of DNA that results from cutting a larger piece of DNA with a restriction endonuclease

technique used to differentiate unlike DNA sequences by cutting the sequences with restriction endonuclease enzymes and visualizing the varying lengths of the resultant restriction fragments

enzyme that uses RNA as a template to make a DNA copy

place in the genome where a single nucleotide is substituted for another

containing recombinant DNA from another species