Mass spectrometry is an analytical method that can detect fragmentation and mass of organic compounds. The ions are passed through a magnetic field that causes them to separate based on their size. The ions then pass through a slit and onto a detector, and a peak is generated on a graph, indicating the relative abundance and weight of the individual components in the sample. The largest peak is usually the peak, which is equivalent to the mass of the molecule. The electron bombardment causes the molecules to undergo further fragmentation, which results in a series of smaller peaks.
High-resolution mass spectrometry measures molecular ion peaks to four decimal places to determine the exact molecular formula of a compound. GC-MS, LC-MS, ESI, and MALDI MS can involve high-resolution chromatography and combine an additional separation step before subjecting samples to mass spectrometry.
At A Glance
- In mass spectrometry, a compound vaporizes and converts to ions, mostly by electron impact. The ions are separated by a quadrupole magnet and then found by a detector.
- In mass spectrometry, parent peaks and fragmentation peaks are generated. Alkanes are fragmented in numerous ways based on their branching.
- High-resolution mass spectrometry measures molecular ion peaks to four decimals to determine the exact molecular formula of a compound.