The ability of an organism to control which genes are transcribed in response to changes in the
A substance that causes mutations. Examples are: certain chemicals and radiation.
A permanent change that occurs i
MolGen Lecture 1:
The Experimental Why:
(1) To know growth dynamics of S. marcescens over a 4 hour period
(2) To find # of CFUs based on measured OD value
-When measuring growth using a spectrophotometer, you need to know what the OD value corresponds to
MolGen Lecture 4:
Chemical mutagenesis-controls: Plate out.
1. Original culture at OD of late log phase
a. We plate this out because we want to know CFU/ml is for the OD chosen and making SURE youre in late
b. Want to know what phase of growth w
MolGen Lecture 5:
Slime found on petri plates could be the ability of the bacteria to tolerate (non-inheritable trait) the antibiotic
Antibiotic was not effective in suppressing growth
For SAME bad medium: slimy mess overnight because you have overgrow
MolGen Lecture 2
How does bacterium grow over time? Shape of growth curve (Sigmoidal). How does growth curve correlate with growth
High cell density to a low cell densityCHANGE TIP! Because there are cells that stick to the outside and inside
enzyme that helps synthesize new DNA molecules
Relatively short piece of DNA synthesized "backwards" on the lagging strand during
DNA replication. DNA polymerase can only go one way and we can't wait until the whole
Group of 3 bases found on tRNA
Transfers amino acids
Makes up ribosomes
Single stranded version of a gene; recipe for a protein
Monomer of a polypeptide
DNA polymerase III
Adds new DNA nucle
What are the three types of RNA?
Messanger RNA (mRNA), Transfer RNA (tRNA) and Ribosomal RNA (rRNA)
What is the purpose of (mRNA)?
A copy of DNA that acts as a template for protein synthesis.
What is the purpose of (tRNA)?
Carries amino acids to (mRNA).
MolGen Lecture 3:
NTG Kill Curves:
Rapid decline with NTG tells us that this mutagen causes a rapid cell death (at high concentration)
Flattening out means NTG concentration has no effect once you get to a saturation point because the cells