An Introduction to Chromatography
What IS chromatography?
The separation of a mixture by distribution of its components between a mobile and
stationary phase over time
mobile phase = solvent
stationary phase = column packing material
A forensic science often referred to as ballistics. Forensic ballistics identifies firearm
usage in crimes.
Its the identification of fired bullets, cartridge cases or other ammunition components
as having been fired from a specifi
Ephoton = h = hc/
Figure 14.52: Schematic representation of two electronic energy levels in a molecule
Figure 14.53/54: The various types of
transitions are shown by vertical arrows.
The science of detecting and identifying the presence of drugs and poisons in
body fluids, tissues, and organs.
Controlled Substances Act
Federal Law established 5 schedules of classification of controlled substances
Introduction to Fingerprint Identification
A fingerprint is an INDIVIDUAL CHARACTERISTIC
no two have yet been found to possess identical ridge characteristics.
The foundation for its acceptance in court
Estimating Time of Death and Postmortem Interval (PMI)
Postmortem Interval (PMI)
The time elapsed from the moment of death until a corpse is discovered is also
known as the postmortem interval, or PMI.
Time of Death
The moment of death
Time of death and P
Basics of DNA
DNA is contained in nucleus of cell
Phospho-lipids and proteins combined to form cell membrane
Lipids are fats
DNA (DeoxyriboNucleic Acid) is a double helix
Backbone is made of sugar-phosphate
Base pairs bind the backbon
Continuous energy is like a ramp.
Quantized energy is like a stair case.
Each stair increases the energy by the value of Plancks constant
h = 6.63x10-34 J-s
E = hf
Neil Bohrs Model of Hydrogen
Solves problem of why electrons do not
Identification and Characterization of Blood and Bloodstains
Apply chromogen (color changing chemical)
Apply oxidizing agent (hydrogen peroxide)
The catalyst of the reaction is hemoglobin
Presumptive Screening Tests
Color Test Method