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5.36 Biochemistry Laboratory
Spring 2009
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5.36 Lecture Summary #6
March 17, 2009
Upcoming CI-M due date: The final draft
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5.36 Biochemistry Laboratory
Spring 2009
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5.36 Lecture Summary #4
Thursday, February 26, 2009
CI-M assignment: The first
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5.36 Biochemistry Laboratory
Spring 2009
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5.36 Lecture Summary #5 (shortened)
Thursday, March 5, 2009
CI-M assignments:
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5.36 Biochemistry Laboratory
Spring 2009
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5.36 Lecture Summary #3
Thursday, February 12, 2009
CI-M workshop on Feb. 19th
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5.36 Biochemistry Laboratory
Spring 2009
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5.36 Lecture Summary #2
Tuesday, February 10, 2009
Next Laboratory Session: #3
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5.36 Biochemistry Laboratory
Spring 2009
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5.36 Lecture Summary #1
February 3, 2009
Reading assignment for Thursday: Weisb
SFM: CELL PHYSIOLOGY
TOPICS: Membrane Potentials
DATE: August 15, 2012
LECTURER: Anita Zimmerman, PhD
The cell membrane is an electrical capacitor in that it is mainly an insulator (lipid bilayer)
separating two conductors (intracellular and extracellular
SFM: CELL PHYSIOLOGY
TOPICS: Cell Membranes and Transport
DATE: August 14, 2012
LECTURER: Anita Zimmerman, PhD
MAJOR TOPICS:
Transport mechanisms
Diffusion
Carrier-mediated transport
I.
Structure and function of the plasma membrane
Epithelial transport sy
MIT Biology Department
7.012: Introductory Biology - Fall 2004
Instructors: Professor Eric Lander, Professor Robert A. Weinberg, Dr. Claudette Gardel
Notes LECTURE 5 Biochemistry 9.17.04
ATP is both a unit of energy currency in a cell and a building block
Biochemistry I Fall Term, 2004
Problem Set 10: Due Wed. Dec. 8 in class, for return on Fri. Dec. 10 (Total points = 2 5 )
A. The following problems in Campbell are recommended:
Chapter 15: 9, 10, 13, 16, 22, 28, 37
Chapter 16: 2, 15, 20, 39-41, 46, 47
Cha
Biochemistry I Fall Term, 2004 Problem Set 9 Answers Wed. Nov. 17
1. (6 points) a) RT at 37C = 2.58 kJ/mol [H+] difference: G = 2.303*RTpH = -2.303*2.58*1 = -5.94 kJ/mol difference: G = ZAF = -1*96.5*0.08 = -7.72 kJ/mol Total G = -5.94 -7.72 = -13.66 kJ/m
Biochemistry I Fall Term, 2004 Problem Set 9: Due Wed. Nov. 17 A. The following problems in Campbell are recommended: Chapter 6: 40-42, 44, 45. Chapter 13: 2, 5, 7, 18, 21, 23, 25, 29, 32, 33, 36. B. Submit answers to the following problems for grading. 1
Biochemistry I Fall Term, 2004
Problem Set 8 Answers
Nov. 10
1. a) (2 points)
The formyl group is attached to methionine in a peptide bond. Thus, fMet-tRNAfMet starts
a polypeptide chain by mimicking a peptidyl-tRNA; its placement in the P-site could be
v
Biochemistry I Fall Term, 2004
Problem Set 8: Due Wed. Nov. 10
A. The following problems in Campbell are recommended:
Chapter 7: 3, 11, 16, 21, 25-27, 31, 33, 38, 39, 49
B. Submit answers to the following problems for grading.
1. (5 points)
a) In bacteria
Biochemistry I Fall Term, 2004 Answers to Problem Set 7 Wed Nov. 3:
1. (3 points) Similarities (between DNA Pol III & RNAP) a) template-directed synthesis b) processive synthesis c) 5'->3' synthesis. d) multisubunit enzymes (10+ for DNA Pol III & 5 for RN
Biochemistry I Fall Term, 2004 Problem Set 7: Due Wed. Nov. 3 A: The following problems from Campbell are recommended: Chapter 10: 4, 6, 8, 13, 15, 16, 20, 21, 32-34, 45 (answer is #46). Chapter 11: 2, 4, 7, 8, 15, 16, 21, 25, 32, 36. B: Submit answers to
Biochemistry I Fall Term, 2004
Problem Set 6 Answers Wed. Oct. 20
1. (5 points)
a) a) First calculate the volume of the cell in liters (using 1m = 10-4 cm):
V = r2h = (0.5*10-4)2(2*10-4) = 1.57*10-12 cm3 = 1.57*10-15 L i.e. 103 cm3/L
Now calculate mol of
Biochemistry I Fall Term, 2004
Problem Set 6: Due Wed. Oct. 20
A: The following problems from Campbell are recommended:
Chapter 8: 2, 3, 5, 11, 13, 37, 39, 44.
Chapter 9: 4, 10, 18, 29-31, 36.
B: Submit answers to the following problems for grading:
1. (5
Biochemistry I Fall Term, 2004
Problem Set #5 Answers
Oct. 13, 2004
1. (7 points) The reciprocals of the [CO2] and vo values are compiled in the table
[CO2]
(mM)
2
4
8
16
32
64
1/[CO2]
1/(mM)
0.50
0.25
0.125
0.063
0.031
0.016
[AZA] = 0
vo (mM/min)
0.88
1.
Biochemistry I Fall Term, 2004 Problem Set 5: Due Wed. Oct. 13 A: The following problems from Campbell are recommended: Interchapter A: 10, 16-18, 22. Chapter 5: 8, 9, 20, 31, 32, 34. Chapter 6: 1, 7-9, 14, 15, 33. B: Submit answers to the following probl
Biochemistry I Fall Term, 2004
Problem Set #4 Answers
Oct. 6, 2004
1. (6 points) ATP Hydrolysis Reactions a) First calculate G' at 37C (310K) using H' = -20.1 kJ/mol and S' = 34.9 J/mol*K (problem 12.17): G' = H' - TS' G' = -20,100 - 310*34.9 (Calculated
Biochemistry I Fall Term, 2004
Problem Set 4: Due Wed. Oct. 6, 2004
A: The following problems from Campbell are recommended:
Chapter 4: 2, 20, 25, 26, 28, 45, 46, 51.
Chapter 12: 2, 3, 13, 17, 20, 22.
B: Submit answers to the following problems for gradin
Biochemistry I Fall Term, 2004 Problem Set 3: Due Mon. Sep. 20 (to receive graded papers by Wed. Sep. 22; otherwise, the due date is in class on Wed. Sep. 22.) A: The following problems from Campbell are recommended: Chapter 4: 4, 9, 17, 21-23,32. (Proble
Biochemistry I 1. (5 points)
Problem Set #2
Answers
Sep. 15, 2004
Statement a) Ala is present predominately as the +1 species. b) The average net charge of Ala is +1/2. c) Half of the amino groups are ionized. d) The pH is equal to the pKa of the carboxly
Biochemistry I Fall Term, 2004 Problem Set 2: Due Wed. Sep. 15A: The following problems from Campbell are recommended: Chapter 3: 2-4, 10-11, 14, 21, 24-26, 38. Answers to these problems are in the back of the text. Please do not submit them for grading.