I. Case 1 Intro. & PAK1.1
Today II. Structure of this course
III. Intro. to Microbial World
Chpt. 1-3 Brock
Case 1 Intro on ELMS
Answer PAK1.1 Questions (ELMS quiz) by 5 p.m. 2/2
Cultivation of Bacteria
In this lab exercise you will be subjecting your Assignment 1 environmental isolates to a variety of different
chemical and physical conditions. It will be important to label carefully all of your plates and tubes so that
Protocols for Slide Preparation, Heat Fixation, Simple Stain, Gram Stain
OVERVIEW & RELEVANCE TO ASSIGNMENT 1:
This lab provides your first opportunity to characterize the organisms in your environmental isolate from the
Winogradsky column. In th
BSCI283 Fall 2016 Exam 1
This is the take-home portion of Exam 1. Pages 2-3 contain some background, and pp. 4-7 are tables of
specific genes, some of which will be useful for answering the five questions which are found on p. 8-9. You
will need to select
DEPARTMENT OF MANAGEMENT
FACULTY OF BUSINESS ADMINISTRATION
UNIVERSITY OF NIGERIA
Names: Ogechukwu Charles Ugbam
State of origin:
3.3A In a period of three months, Fredrica has contracted three different
diseases caused by Streptococci. Why didnt her recovery from the first
streptococcal infection give her long-term immunity? What is the likelihood that
she will get another infectio
3.2A Why did Fredrica's pediatrician order a lab analysis of her white blood cell
White blood cell counts can detect infections in bodies and alert doctors to
medical conditions. A high white blood cell count usually indicates increased
These are the ALQ9 questions.
Put your answers in your ELN in the As2 Day 5 tab. Dont repeat the questions there.
Due at beginning of lab April 3 or 4.
Use the data in the table below to answer the questions. These data are for OD readings made using
These are the ALQ7 questions.
Put your answers in your ELN in the As1 Day 6 tab. Dont repeat the questions there.
Due at beginning of lab March 8 or 9.
In the Microbial Olympics Lab, you used some strains of Chromobacterium violaceum, including a strain
3.1A Fredrica has an ear infection caused by Streptococcus pneumoniae.
Why doesnt she have pneumonia?
The symptoms that are portrayed due to infection with these bacteria depend on
the part of the body that is infected. It is usually present in the respi
The Bacillus thuringensis strain called kurstaki includes an operon on its genome that encodes
for the insecticidal toxin, CryB1. The cryB1 gene was the one that was introduced into corn to
create the first versions
Final Exam Take-home Cumulative Questions
There are 9 questions for this exam.
To answer these questions, you may use whatever sources you find helpful. While you may work in groups to
answer these questions, your answers must be in your own words.
Agrobacterium tumefaciens is a bacterium that can infect wound sites in many different plants, causing
a disease called Crown Gall in which the bacterium genetically reprograms plant cells such that they
lose their normal control mechanisms and grow into
Inactivated Polio Vaccine has Diminished Polio Cases
A Brief Introduction to Paralytic Polio Disease: Polio is a frightening disease that can lead to irreversible
paralysis and even death. It is caused by a virus that enters the body through the mouth and
Rise of the Wrinkly Spreaders
Introduction and Overview
This is an extended lab exercise that will give you hands-on exposure to some key aspects in
evolution as they apply to microorganisms: spontaneous mutations, niche colonization, competitive
Fall 2014 BSCI283 Exam 1 Take-Home Questions
Your Lab Section/UTAs name:
Open book, open notes, open internet.
You are encouraged to work on these questions together with other class members!
BUT your final answers must be written
A very detailed diagram of an E. coli cell
In a living cell (E. coli) there are.
2,400,000 protein molecules
250,000 rRNA + tRNA molecules
50,000,000 metabolites (small molecules)
250,000,000 inorganic ions
25,000,000 lipid molecule
Lecture 9: Microbial Growth
Different microbes have different anabolic and catabolic
abilities that determine nutritional requirements for growth. pp. 73-102
o Planktonic vs. Biofilm growth
pp. 143-180, 597-605
o Microbes in lab vs. na
Antibiotics Part II
Reading: For lectures on symbiosis read Chapter 22, for lectures
on natural products and antibiotics read the second half of Chapter
27 (811-825), for portion of lecture on biofilms, read pages 600-607
The mathematics of uncontrolled growth are frightening. A single cell of the
bacterium E. coli would, under ideal circumstances, divide every twenty minutes. That
is not particularly disturbing until you think about it, but the fact is that bacteria
We live on the Planet of Bacteria!
Article by Stephen Jay Gould
(Professor of Geology and Zoology at Harvard University)
Bacteria are - and always have been - the
dominant forms of life on Earth.
The Operon Model of gene
regulation, as proposed by Francois
Jacob and Jacques Monod
Due Next Wednesday: PAK2.2
Anyone with a computer and internet access can look
Chapter 13 on Metabolic Diversity
Chapter 22 on Microbial Symbioses
Next Case Study: To be introduced soon!
Assignment #2: Ill briefly discuss it at the beginning of
Stationary phase growth
no net increase in
Antibiotics Part I
The Lord hath created medicines out of the earth; and he that is wise
will not abhor them.
Ecclesiasticus, XXXVIII, 4
A recent example of a new natural product with
antibiotic activity; Closthioamide; Discovered by
Aseptic Technique and Streak Plating
TO DO TODAY
1. Learn and practice aseptic technique (Protocol 1)
2. Dilution streak a plate to obtain individual colonies (Protocol 2).
Streak Plates and Morphologies of Colonies on Plates
Big G, Little :
Using your compound light microscope to observe a wet mount
Each bench has a little plastic dish containing some small slips of paper. Each of you will
need one. They are not identical to one another. Each slip of paper looks like the diag
Initial Steps With Your Environmental Samples: Isolating Pure Cultures
Protocols for Dilution Plating, Streaking for Isolation, and Aseptic Tube Sampling
OVERVIEW & RELEVANCE TO ASSIGNMENT 1:
One major goal today is for you to start isolating microbes fro
Protocols for use of the Light Microscope, Preparation of Wet Mounts and Tape Mounts
Overview: this lab will require two periods. You will use your bright-field student microscope to
examine unstained samples.
o During PERIOD ONE, become fami
Reading: For lectures on symbiosis, natural products and
antibiotics, read Chapter 22 and the second half of Chapter 27, for
portion of lecture on biofilms, read pages 600-607 (in Chapter 19).
I will introduce Case Study #2 at the start of the