The objects of this experiment are to introduce students to a voltage regulator system,
voltage regulator concepts, and the voltage regulator integrated circuit chip (IC).
Students will learn how to calculate voltage regulation, how to design a vol
Polymerase Chain Reaction
(PCR) and Its Applications
Department of Molecular Biology and Genetics
What is PCR?
PCR is an exponentially
progressing synthesis of the
defined target DNA sequences in
It was invented in
Why Extract DNA?
Purified DNA ready for direct use in downstream
Removes proteins, nucleases and other impurities
and any potential PCR inhibitors (EDTA, FFPE,
More stable/less degradation/easi
Ribosomes and rRNA
75 to 90 nt (bact and
eu/k tRNA almost
In both o/m, tRNA
are transcribed as
cleaved into mature
Transcription of DNA to
obtain rRNA and tRNA in
1. Which of the following is NOT a way in which DNA is different from RNA?
A) The nitrogenous bases.
B) The number of strands.
C) The kind of sugar in the sugar-phosphate backbone.
D) All of the above are ways in which they differ.
Use the terms below to
Dominance is not universal
(not following Mendel)
If dominance were universal,
heterozygote cross always give 3:1 ratio.
If heterozygotes were distincly different
from both homozygotes, a 1:1 ratio of
phenotypes occurs when heterozygot
mRNA information for aa seq.
tRNA specific aa for each tRNA.
ribosome workbench for translation
aa joined to 3-end of
tRNA charging @
Regulation Of Gene
Bact regulates synthesis of gene
products (expression) by switch
genes on and off depending on cells
Some protein are produced
continuously constitutive. Eg:
Bioinformatics lab 1: Tutorial to Basic
Bioinformatics: COX-2 (PTGS2)
Getting sequence information and viewing database entries NCBI-gene
Step 1: Go to the NCBI homepage
Step 2: Select Gene from pull down menu.
Step 2: Ty
DO WHAT THE FUCK YOU WANT TO PUBLIC LICENSE
Version 3, December 2013
Copyright (C) 2013 mca64
Everyone is permitted to copy and distribute verbatim or modified
copies of this license document, and changing it is allowed.
DO WHAT THE FUCK YOU WANT TO
Battle.net account email: firstname.lastname@example.org
Current name registered on account: eer dsfdsf
Last 4 digits of credit card number: 6657
When I first created this account, I entered a random name and country of residence because I didn't think it mattered
is pregnant, but
whos the daddy?
How can we find
A real problem for a real
Johanna Nielsen is a PhD student at
the University of Edinburgh and the