Chapter 12: Enzyme Kinetics
Everything In This Lecture Is Important!
Basics of Chemical Kinetics
First and Second Order Reactions
Km, kcat, Michaelis-Menten Equations
3304 Final from Last Midterm
Pentose Phosphate Pathway
Alternative Hexose Metabolism
Citric Acid Cycle
Be able to write these pathways, enzymes,
intermediates in detail from memory.
Know the mechanism basics for all of the
Lecture 20 Cheat Sheet
1. Oxidation and Reduction.
2. NAD+ to NADH
3. FAD to FADH
4. Differences between NAD+ and FAD+
5. Nernst Equation
DG = - nFDE
6. Half cell potentials and how they relate to electrochemical
7. Experimental approaches for
Lecture 19 Cheat Sheet
1. Metabolism definition.
2. Catabolism and anabolism in metabolism.
3. Areas where metabolism occurs:
a) Oxidative phosphoryolation
b) Carbohydrate metabolism (Glycolysis)
c) Fatty acid and lipid metabolism.
Glycolysis = Rapid ATP Production
While oxidative phosphorylation in mitochondria can
produce up to 32 ATPs per glucose, glycolysis
(producing only 2 ATPs per glucose) is about 100 times
Fast twitch muscles - short blasts of energy and are
Lecture 22 Cheat Sheet
Only way to do this is memorization!
Glycolysis: Know all steps, intermediates and enzymes
Basic idea is to phosphorylate glucose to activate and
progressively break down internal bonds.
Points to Assist in Memorization:
Stage I: Go