Acute Aspirin Overdose:
Relationship to the Blood Buffering System
The response of the carbonic acid/bicarbonate buffering system to an overdose of aspirin is examined.
Principles of acids and bases, including pKa an
Inhibition of Alcohol Dehydrogenase
The inhibition of the alcohol dehydrogenase by a formamide compound is examined.
Principles of enzyme kinetics
Identification of inhibition via Lineweaver-Burk plots
One can apply the H-H equation to the amino and the carboxyl group each
in turn, then multiply the results to arrive at a ratio of 10-5.
First, consider the amino group, pH = pK +log [NH2]/[NH3+]. With pH=7
and pK=8, on has 7=8 + log [NH2]/[NH
Bio40 Problem Set 3
Problems on Enzyme kinetics and mechanism
(note: Michaelis Menten equations are given in the powerpoint presentation on Enzyme kinetics,
as well as in the textbook. You will be given the Michaelis Menten equations on the exam
BIOL40: Problem Set 2 (lectures 4- 8)
1. Charged compounds such as amino acids can be separated by taking advantage of their
different mobilities in an electric field.
A rapid estimate of the effective charge on an amino acid can be made by comparing its
BIOL40: Problem Set #1
Lecture 1: Introduction
Text Chap 1: #1-4
1. Identify the functional groups in aspartic acid. In proline. In coenzyme A.
2. A spheroidal bacterium with a diameter of 1 um contains 100 molecules of ATP. What is the
Acute Aspirin Overdose: Relationship to the Blood Buffering System
2. These questions can be answered by using the Henderson-Hasselbalch equation:
At pH = 2, the percentage of salicylate (unprotonated) is 9% (0.1/1.1) and the percentage of sa
Allosteric Interactions in Crocodile Hemoglobin
The effect of allosteric modulators on oxygen affinity for crocodile hemoglobin is unique when
compared with other species.
Hemoglobin structure and function concepts.
BIO40 REVIEW FOR EXAM 2
Memorization required (briefly):
Amino acid structures (but your already know these, right?)
Lipids (nomenclature, common names for lipids)
why are enyzmes used; what are c
BIO40 Sample Exam 2 questions
Multiple Choice Questions
_1. The pK of the R-group of Asp will _ when surrounded by negatively charged
C. Remain the same
_2. The main difference between a catalyzed and an uncatalyzed react