Two factors determine whether a molecule will cross a membrane:
The permeability of the molecule in a lipid bilayer
Lipophilic molecules can dissolve in the lipid bilayer
Simple diffusion down their concentration gradient
Double-displacement (Ping-Pong) reactions:
One or more products are released before all substrates bind the enzyme.
Substituted enzyme intermediate the enzyme is temporarily modified.
Example: reactions that shuttle amino groups between am
Page 229-237, 246
Describes the kinetic (the rates of chemical reactions) properties of many enzymes
First-order reaction reactions are directly proportional to the reactant
First-order rate constants have the uni
Chapter 12 pp.345-355
Biological membranes: boundaries of all cells
Prevent molecules generated inside the cell from leaking out and unwanted
molecules from diffusing in.
Transport systems that allow the cell to take up specific molecules and
Chapter 8 p219-229, Chapter 9 p253-263, 266-271
Catalytic power - active site depends on cofactors.
Cofactors are able to execute chemical reactions that cannot be
performed by the standard set of twenty amino acids.
Apoenzyme + cofactor = holo
Hemoglobin: Portrait of a Protein in Action
Vertebrates-supply cells with an adequate supply of oxygen:
1. Circulatory system-actively delivers oxygen to cells thought the body
2. The use of the oxygen-transport and oxygen-storage proteins, hemoglobin
CHEMISTRY DEPARTMENT DREW UNIVERSITY 7/26/11
LABORATORY AND STOCKROOM SAFETY PROCEDURES AND RULES
Everyone must wear protective glasses with side shields at all times in the laboratory. An appropriate type can be purchased from the stockroo
Materials and Methods
A number of steps were required in order to analyze the activity of YADH from the
yeast species Saccharomyces cervisiae. Before testing the ADH with assays in
order to determine enzymatic activity and a number of other variables, t