Principles of Microbiology
STUDY GUIDE 2:
1. Define bacterial growth, including binary fission.
a. Growth in number, exponentialy grown and die. Binary Fission is when a
Prokaryote replicates its circular DNA then splits.
2. Classify microorganisms into t
Lab # 6
Aseptic Technique and Isolation Streak
A good aseptic technique is the key to keeping an inoculum contaminant free and
yielding correct result. To practice aseptic technique we performed an isolation streak
plate of two organisms. The purpose of t
Study Guide: Exam I
1. Identify the contributions to microbiology made by van Leeuwenhoek, Hooke,
Pasteur, Koch, Lister, Fleming, Jenner.
1. Hooke living things were composed of boxes or cells
2. Leeuwenhoek- observed live microorganisms in teeth scraping
PRACTICE EXAM: It is recommended that you study before taking this exam. This
will enable you to assess your strengths and weaknesses more effectively. I wont
provide an answer key. Use your notes, text and especially peers to make sure you have
August 15 2012
Unknown Bacteria #1
Our final lab in class was identifying an unknown bacterium. The end of every lab
prior has yielded some sort of information about the known bacteria we were handling. In
turn the final lab is
MRSA GENERAL INFORMATION
What is it?
Whats the MR for?
Where is it?
Am I safe from it?
Ex. 16 and 17 Acid Fast Staining and Endospores
Acid fast is another stain that differentiates two types of bacteria, acid fast and
non acid fast. Acid fast cells have a waxy cell wall making it resistant to desiccation and
disinfectants, also making it d
Ex. 10 Fungus Among Us
Fungus is tricky because it helps and harms humans at both extremes. Fungi
provide us with antibiotics in medicine but are also infections like athletes foot. This
eukaryote is both friend and foe, but most importantly, it is a deco
Ex. 12 Simple Stain
A simple stain uses either an acidic or basic stain to make all the cells on your
slide one color. If an acidic stain is used then only the background gets colored, and this
is called a negative stain. The purpose of this lab was to do
Ex. 15 The Gram Stain
Hans Gram figured out a stain that differentiated the two major groups of bacteria,
those with a thick layer of peptidoglycan and those with a thin layer. Cells with a thick
layer are Gram positive; cells with a thin layer are Gram n
Ex. 5 Microscopes
The goal of our first lab was to get comfortable with the microscopes. We learned
proper technique and how to prevent them from breaking. The purpose of the lab was to
view the specimens under 1000X magnification using immersion oil.