Chapter 5 - Problems
Book (Ch. 5, prob. 4a)
4a) In what order would the amino acids Arg, His, and
Leu be eluted from a carboxymethyl column at
pH6?
The most frequently used cation exchanger is: carboxymethyl (MatrixCH2-COO-)
At pH6: Arg(+1), His(+0.5), L
Chapter 12, Problem 2
If there are 10 M of radioactive 32P (half life 14 days) at t=0, how
much 32P will remain at (a) 7 days, (b) 14 days, (c) 21 days and
(d) 70 days?
For a first order reaction (decay) [A] = [A]o e-kt
and t1/2 = 0.693/k
k = 0.693/14 d
Chapter 11, Problem 6
Approximately how much does staphylococcal nuclease
(Table 11-1, rate enhancement = 5.6 x 1014) decrease the
activation free energy (G) of its reaction (the hydrolysis
of a phosphodiester bond) at 25oC?
The free energy associated w
Chapter 6 - Problems
Book (prob. 2a)
What is the NM notation for an -helix?
N = the number of repeating units per turn
M = the number of atoms that complete the cyclic system
that is enclosed by the hydrogen bond.
The NM notation for an -helix is 3.6
Chapter 5 Problems
Book: 1, 3, 4, 6-8, 12, 14-20
Book (Ch. 5, prob. 15)
Cleavage of a polypeptide by CNBr and chymotrypsin yields
fragments with the following amino acid sequences. What
is the sequence of the intact polypeptide?
CNBr treatment
Chymotryps
Chapter 4: Problems
Book: 1, 4-10, 12, 13, 16, 17
Book (Ch. 4, prob. 4)
Draw the dipeptide Asp-His (DH) at pH 7.0
N-term=9.40, Asp=3.90, His=6.04, C-term=2.20
N
HN
H
O
NH3+
-OOC
N
CH2
H
H
Acid:
If pH<pKa, neutral (0)
If pH=pKa, (50% ionized, -0.5)
If