Is there a promoter on mRNA?
Amino acid: all proteins are composed of amino acids (20).
Common AA are similar in structure
Structure and function of proteins
a _ consist of three nucleotides
_ different codons make _ amino aci
Determining the order of the nucleotide bases-A,G,C, and T- in a
molecule of DNA
The original sequencing method is_
1) can be added
Uses for Cloned DNA
1) molecular probe
2) sequence the DNA
3) express the cDNA to make protein
One of the first steps in characterizing a DNA clone is to construct a
indicates the number, order, and distance between restrictio
Agrobacterium-mediated plant transformation
1) In natural gene transfer, the Agrobacterium invades the plant at a wound.
2) Part of the Ti plasmid is transferred to the plant cell
3) where it integrates into one of the plant chromosomes
What is the Central Dogma of Molecular Biology?
DNA is transcribed to mRNA and translated into polypeptide (protein).
_ is the process by which a molecule of DNA is copied
into a complementary strand of RNA.
DNA can be
Any genetic material must be able to do these 3 things?
3. Store and express information
The nucleus predominately contains?
Which is the genetic material?
Nucleic Acids:DNA and RNA
able to cause disease
Process of copying DNA, occurring in all living organisms
What are the three possible mechanisms of replication?
Which do we use?
Who discovered that we use the semiconservat
This reflects the rate at which a molecule sediments under the
centrifugal force of a centrifuge
(S) is measured in Svedberg units (S = 10E-13 sec)
Three rRNAs in prokaryotic ribosomes
5S, 16S and 23S
5S and 23S belong to large subunit, 16S bel
Cells don't express all of their genes all of the time.
Gene expression varies:
between different tissues
between different environments
Much of the gene regulation in prokaryotes/Eukaryotes is accomplished
Breeders choose plants and animals with the most desired traits to serve
as parents of the next generation.
Increasing the frequency of desired alleles in a population is the
essence of genetic technology.
mating between cl