Both Dot and Western blot techniques provide detection of protein and nucleic
acids, which are similar pieces of information.
Upon reading the steps to perform a western blot, ELISA technique I thought would
provide similar information.
Pre Lab 3/27/2015
1) A reasonable hypothesis for this weeks lab that would reference all possible
questions could be that phagocytosis of opsonized cells will be more efficient
than those that are not. Another possibility is that free partic
1) Outline the major steps for how TLR 4 might activate SAPK pathway (note the
intracellular adaptor, kinase signaling cascade, transcription factor and final contribution
that aids the immune system).
To be discussed in lecture
Bio 350 Lab
Pre-Lab 3 Macrophage Phagocytosis Assay
1) In innate immunity the function of phagocytes are to trigger
defenses against invading pathogens; divided into dendritic and
macrophages are the first immunological cells to in
Bio 350 Lab
Pre Lab Histology
1) Bone marrow, thymus, spleen, lymph vessels and nodes, other lymphoid
tissues (MALT, Peyers patches, tonsils, adenoids)
2) The most important difference between primary and secondary lymphoid
Histology Pre Lab 1
1) The four major components of blood are plasma, red blood cells
(erythrocytes), white blood cells (leukocytes), and platelets (thrombocytes).
2) The major functions of cells found within the blood are o
Virtual lab questions
Slide 140- 141 Thymus
Slide 140 is a natal thymus as it displays a darker color and is low in adipose tissue.
Slide 141 is an adult thymus displaying significant adipose tissue, involution,
Bio 350 Lab 2
Introduction to cell culture and sterile techniques
In the practice of sterile technique it is important to consider:
- use 70% ethanol in order to maintain a contaminant free environment
- use the plastic on the pipe