Lab Two Homework Assignment
You are extracting DNA from 10 ml of whole blood. WBCs (white blood cells) are
collected via centrifuge. WBC concentration is 8x10^6 cells / ml. Answer the following
How many WBCs can you collect?
Lab One Quiz
Question: At which stage of meiosis is the production of female gametes in humans arrested
Answer: Production of female gametes is arrested during prophase I of meiosis.
Question: Why is fertilization in angiosperms called doub
Genetics Lab: BISC-2549
Lab 5: Miniprep Analysis of Transformants; Bioinformatics and
* Miniprep analysis of transformed plasmid
Bioinformatics and Phylogenetics
Course materials on Blackboard.
Part 1: Analysis of Transformants
Part II: Bioinformatics and Phylogenetics
Review from Lab #4
We transformed JM109 E. coli bacteria using
a plasmid containing our DNA insert and then
plated the bacteria on selective media
What does it mean to tr
Restriction Enzymes, PCR, and
Run restriction enzyme digest on lamba
Lecture Restriction Enzymes, PCR, Gel
Set up PCR reaction using calf thymus
We isolated DNA and determined its
DNA Extraction Part II
1. Finish DNA extraction
1. Determine concentration of DNA (use
procedure from manual starting with step
16 on page 161). See last weeks
handouts or manual page 159 for a good
description of how this works.
Lab Number Three: DNA Extraction Part II
Protocol for determining DNA concentration: (1) Each paid of tables will make a
standard curve by labeling 1.5 ml tubes 1 through 5. The samples for the curve
will be made by making a series of two-fold dilutions
Lab Number One: Mitosis and Meiosis
Preparation of onion root tip: (1) obtain two small cups. Label one HCL and
pour enough HCl into it to cover the bottom. HCl softens the root tips and
separates the cells into one later. (2) Likewise, label the other C
Lab Number Two: DNA Extraction Part I
Procedure for extraction of DNA from thymus: (1) Obtain a small piece of thymus
from the instructor. Cover the tissue with a small amount of 1x PBS to keep the
tissue moist. Use a razor to remove the white connective
Mitosis and Meiosis; Barr Bodies (Brief Review)
Mitosis: one stage of the cell cycle. The other stages of the cell cycle are G1 (gap
one), S (synthesis), G2 (gap two), and Mitosis.
Mitosis is the duplication of chromosomes and cellular division (somatic
Lab Three Homework Assignment
What is Beers Law? How can it be used to predict DNA concentration?
Beers law states that there is a direct relationship between the optical absorbance
(A) of a compound and its concentration (c): A = kc. This law can be use
Lab Number Four: PCR, Restriction Enzyme and Electrophoresis
Digestion of lambda phage DNA and calf thymus genomic DNA: (1) Obtain 4
tubes that are 200 microL size per table and label them L/E, L/H, G/E, and G/H. L
stands for lambda phage, G stands for c
Chapter Fifteen: The Dynamic Genome: Transposable Elements
Transposable elements are also
known as mobile elements,
jumping elements, jumping genes,
junk DNA, selfish elements etc.
They can move to new positions
within the same chr
Chapter 10: Gene Isolation and Manipulation
Until the early 1970s, DNA was the most difficult biological molecule to analyze.
Today DNA has become the easiest macromolecule to study.
It is now possible to:
(1) Isolate a specific region of almost any gen
Chapter Eleven: Arabinose and
operon is an example in
which a single DNA-binding
protein (AraC) may act as either a repressor or an
Transcription is activated at araI: the
initiation region (contains a binding site
Chapter Five: Bacterial Genetics
Bacteria are the most numerous organisms on our planet earth. They contribute to
recouple of nutrients such as nitrogen, sulfur and carbon in the ecosystem.
Some bacteria are agents of human, animal, and plant disease. O
Chapter Twelve: Regulation of Gene Expression in Eukaryotes
Gene regulation in eukaryotes is more complex than it is in prokaryotes. In
eukaryotes, most genes are not found in operons, the proteins and DNA sequences
participating in eukaryotic gene regul
Lab 1: DNA Extraction, Part I
You are extracting DNA from 10 mL of whole blood. WBCs (white blood cells) are collected via
centrifugation. WBC concentration is 8 x 106 cells / ml. Answer the following questions.
How many WBCs can you collect?
(8 x 1