MCB 305, Cell Biology
Study questions for Exam 2
This is not meant to be a comprehensive study guide for the exam. I think it works best if
you try to write out your answers, as if this were an
EXAM ONE Study Questions MCB 410 Spring 2011 Lecture 2 1. What are the three main branches of life? - Bacteria (eubacteria). - Archaea (archaebacteria). - Eucaryotes. 2. Name six organelles in the cell and describe their primary function. 1. Chloroplast (
MCB 410 Spring Study questions on cell adhesion, ECM and transplantation. Describe the major functions of tight junctions, gap junctions, adherens junctions, desmosomes,hemi-desmosomes, and focal adhesions. Give an example of where each of these occu
Biochemical Energetics (A Quick Review)
Kinetic energy: energy of motion
o Heat energy
o Radiant (light) energy
o Electrical energy: moving electrons or ions.
Potential energy: stored energy
o Energy in covalent bonds
o Energy stored in a concentration
Fill in Blanks and Short Answer Exam
Sample Exam : 1st Quarterly Examination
Fill the blanks (5 points)
In DNA replication, coordination between (1 leading ) and lagging strand
synthesis is achieved by the dimerization of (2 DNA ) polymerase molecules at
1. The inner and outer nuclear membranes form a continuous sheet, connecting through nuclear pores. Continuity implies that membrane proteins can move freely between the two nuclear membranes by diffusion. Yet, the inner and outer nuclear membranes have d
MCB 410 Spring 2001
Page 1 of 3
MCB 410 SPRING 2001 KEY Second hour exam. March 30, 2001 AVERAGE = 64.3 SCORE DISTRIBUTION 1. (6 pts) Antibodies have two antigen binding sites. Treatment of cells in culture with an antibody that binds to a specific
MCB 410, Spring 2015
Study Questions for Exam 1
Topics: Membrane lipids, membrane proteins, membrane transport, and the
These questions are designed to help you test your understanding of the
Multiple Choices (THE ANSWER IS ONLY ONE). 1 point /Question
1. An approximate size of mitochondrion is 200 nm. What is an approximate size of
A. 1 m
B. 600 nm
C. 6 nm
D. 0.1 nm
2. What is Saccharomyces cerevisiae?
MCB 410, Cell Biology
1. (10 pts) Membrane fluidity must be maintained within a rather narrow range.
a. (5 pts) Please describe, in words and/or using labeled diagrams, TWO
changes cells ca
The precise copying of DNA sequences in preparation for cell division.
Using the existing (parental) strands as templates in the formation of new (daughter)
strands that are complementary to the parental strands.
Defects in DNA repair mechanisms are thought to be linked to cancer.
Mutations accumulate in cells DNA.
-If this affects genes that regulate cell division, cells will divide uncontrollably,
Begins with the polym
The Central Dogma of Gene Expression
Replication, Transcription, Translation
tRNAs (3%), mRNAs (2%), rRNAs (~95%)
The Central Dogma Played Out in the Eukaryotic Cell
READ this figure legend!
A closer look at Nucleic Acids
A nucleotide consists of:
Signaling at the Cell Surface
Signal Transduction: Overall process of converting extracellular signals into intracellular
Signaling molecule= ligand
-binds to a structurally complementary protein on the extracellular domain, called
Protein Structure and Function
Proteins are the most diverse of the macromolecules in terms of structure and
A proteins three-dimension structure is called its conformation.
Correct folding is absolutely critical for protein function.
Describe the two different ways that enzyme-linked receptors can be activated.
Outline the MAP kinase pathway downstream of rTKs. What are the targets of this pathway?
What pathways control the release of Ca+ in cells. The total calcium in cells is
HOMEWORK 4 CELL ADHESION AND ECM KEY 2 revised 4/3
This figure shows cells from the developing liver and developing kidney of an embryonic mouse. The cells have each been dissociated in culture medium without Ca+. When they are mixed back together i
MCB 410 SPRING 2002 First Hour Exam February 8, 2002
1 (16pts). Proteins are often made up of multiple distinct domains. A. (8pts) What is meant by a STRUCTURAL protein domain? Give two specific examples. (4pts for definition; 2pts for each example)
NAME: _lien lai_ 1. (3 pts) Whenever vesicles fuse with target membranes their v-SNAREs are transferred to the target membrane t-SNAREs. For an actively secreting cell this could eventually lead to an accumulation of v-SNAREs in the plasma membrane. How m
1. Where are proteins synthesized? On ribosome which are located in the cytosol 2. What is the fate of a protein with no sorting signal? Remain as permanent residents in the cytosol 3. How would you determine a signal sequence and prove that it functions
Steady State Reactions
Ok, now we have a dissociation reaction:
o GA G + A
o [G] [A]/[GA] = Keq which again does not change
If you add excess A to the reaction, [A] rises.
But, Keq must stay the same.
Therefore, [G] must decline
If the machinery required for protein synthesis (mRNA, aminoacyl-tRNAs, etc.)
were free to float about in solution, the likelihood that they would interact and
assemble would be extremely low.
Protein synthesis rate would be too low to sustain
Question 2 Which of the following happens when sperm fertilizes a mammalian egg? a A) The sperm acrosome reaction digests part of the zona pellucida a C) Sperm nucleus enters the egg
a B) Sperm centriole enters the egg to form the