Lecture 13 Biotechnology
Define biotechnology, genetic engineering, recombinant DNA technology,
GMOs, transgenics and other terminology (vectors, restriction enzymes, etc)
Identify the roles of vectors and restriction enzymes in ma
Lecture 15 Viruses
Do you know what virus is in
the TEM image below? Hint:
Requires scientists to work in
Biosafety level 4 labs (maximum
biological containment, high risk).
Five strains (Ebola-Zaire, Sudan, Reston, Cote dIvoire,
Principles Operation of DSSCs
Polymer and Color Chemistry
Basic operations of DSSCs
For the design of efficient dyes for DSSCs,
five requirements should be satisfied:
1. anchoring groups, e.g. COOH, SO3H or
PO3H2, must exist not only to graft the dye
Lecture 12 Microbial Genetics
Define mutation, mutagen, spontaneous mutation.
Discuss the various types of base substitution mutations and
what effects they may have on the cell.
Discuss why genetic recombination in bacteria is im
Isolation of a novel strain of Bacillus from soil
The purpose of this class, Microbial Diversity, was to
explore the diverse nature of the prokaryotic world
via experiments designed to screen for isolates of a
particular specification. One
Case Study 1: Life Beneath the Ice
1) Using epifluorescence microscopy, the scientists examined the Lake Whillans water and
sediment samples. Figure 3.
a) What is epifluorescence microscopy? For what purpose was this technique used for
in the scientific p
Lab 6 Notes: Enumeration of Viable Bacteria
Common methods to determine the number of organisms in a sample of material:
o Direct microscope count
o Culture turbidity
o Dry weight of cells
o Viable cell count (like this lab)
Most common method is the pl
Lecture 13B Microbial Regulation
of Gene Expression
Define operon and describe its basic structural components.
Explain the regulation of gene expression in bacteria by
Negative (repression and induction) and posit
Structure and Function
TEM ~2um long
Compare and contrast the overall cell structure of prokaryotes and
Recognize the different types of prokaryotic shapes, arrangements, and
Anaerobic Respiration & Fermentation
Compare and contrast aerobic and anaerobic respiration.
Explain how bacteria can support respiration using electron
acceptors other than O2.
Describe how the process of denitrification
Control of Microbial Growth
Identify the roles played by early pioneers in discovering
chemical control of microbial growth (Lister, Semmelweis,
Define the following key terms related to microbial control:
The Flow of Genetic Information
Describe how genetic information flows.
Discuss the role of each type of RNA found in a bacterial cell.
Describe the basic structure of RNA polymerase and understand the role played by the s
Lab 5 Notes
Sulfide Indole Motility (SIM)
o To identify members of the Salmonella and Shigella groups
o Shows hydrogen sulfide production, indole production, and motility all in the same tube.
o Identification within the Gram negative enteric group