Techniques and Theory
Polymerase chain reaction
PCR has the ability to amplify a single
transcript one-billion fold
The resultant amplification can then be
visualized and sized on agarose gel
containing a DNA binding dye, i.e.
Lab 1 Follow up
Working with RNA Dos and Donts
Avoiding ribonucleases - RNAse
Always wear gloves - Skin is an abundant source of ribonucleases.
Introduced during working procedures
Prepare solutions for RNA work using autoclaved glassware, then
Lab Report Rubric
I. Title (1 sentence) (5 pts)
_Clearly summarize the entire lab in one sentence.
II. Introduction/Background (1-2 paragraph(s) (10 pts)
_Describe the question under study (what you are doing and why). Does the expression MT change in res
BIT465/565 : Real-time PCR techniques
An introduction to real-time PCR technologies including
methods to measure gene expression, enumerate organisms,
and genotype single nucleotide polymorphisms.
Dr. Chris Ashwell
Proposal AND lab Report is due next Friday
Lab manual due Monday
Much more of allele 1 than allele 2 - Lab 5 results
SYBR - Assay design
Copy # Ct value