55 points 5.5 = 10 points
Laboratory Report (SS 2013) Exercise Five
TA_1pt_ Lab Section_1pt_
Data (Note this data will also be entered into the Lab computer for class results.)
Genus & species of Known Organism:
Laboratory Ex 6: Bacterial Metabolism II and Identification of Unknown (SS 2013)
Objectives: After completing this lab you should understand:
1. How a variety of physical and metabolic properties of organisms are used to
identify unknown organisms.
Laboratory Exercise 4: Culture Techniques ( SS 2013)
1. Perform basic aseptic procedures of microbiology.
2. Understand some of the different ways of culturing microorganisms and viruses.
3. Have an appreciation for the ubiquity and
50 pts 5 = 10 points
Laboratory Report Exercise Nine (SS2013)
T A_1pt_ Lab Section_1pt_ D ata:
JUST LOOKING FOR TRENDS, NOT SPECIFIC NUMBERS!
HEAT> Evaluate Amount of Growth: 0 to 4+
YOU W ILL NEED TO USE EACH NUM BER M ORE THAN ONCE!
Study Guide for Biochemical Tests (as of 2/04)
30 points 3 = 10 points total
Laboratory Report Exercise Seven (SS2013)
TA_1pt_ Lab Section_1pt_
Data: Also attach your data sheet from the Rapid ID method to this lab report. 3pts
Rapid ID Methods Demonstration: Summary of Results
Lab 5: week 2: RESULTS
In this exercise, you will examine each of the biochemical tests performed on your known
organism and interpret it as 'positive' or 'negative'. Record these results on your Lab 5 report. The
results for all five organisms will also
Lab Six, Week 2: Results of Unknown
A. Identification of Unknown:
1. Observe each test and interpret the results of each biochemical test and record it in Lab report
6. Remember to use your notes from lab 5! Remember you will NOT be allowed to ask a TA
Laboratory Exercise 7: Rapid ID Methods (SS2013)
1. Use biochemical test results to identify an unknown bacterium
2. Understand the use of commercially available rapid-ID kits
In the last two exercises, we have seen how a set of bi
Laboratory Exercise 8: Chemical Antimicrobial Treatments (SS2013)
Objectives: After completing this exercise you should be able to:
1. Evaluate the effectiveness of common household disinfectants/antiseptics.
2. Explain why (and how) phenol coefficients a
Laboratory Exercise 9: Physical Antimicrobial Treatments (SS2013) DEMO ONLY
1. Understand the need for different methods of sterilization.
2. Compare the bactericidal effects of heat on vegetative bacterial cells & endospores.
3. Understand th
o Can barley be seen under the light microscope
o Transmitted to humans by arthropod vectors
o Half the size of rickettsia
o Can not be seen with the light microscope
o Smallest of bac
Laboratory Exercise 3: Special Staining Techniques (SS 13)
O bjectives : Know the basic aspects of endospore, capsule, and acid-fast staining and how bacterial
cells respond in each procedure.
B ackground: O ther Differential Stains
T he acid fast stain i
L aboratory Exercise 2: Staining Bacterial Cells (Spring 13)
1. Use basic methods of "aseptic technique", and understand why it is of vital importance
2. Make and heat-fix a smear from liquid cultures, and understand the re
L aboratory Exercise 1: Basic Microscopy (Spring 13)
1. Learn and practice safe laboratory procedures
2. Understand the principles behind and operate a compound light microscope
3. Prepare and observe a wet mount
4. Observe the morphological d
Lab 5 week 2: Bacterial
Metabolism of Known
2. Positive or Negative?
Semi-solid medium of thioglycolate and
Thioglycolate consumes oxygen, which is
constantly diffusing down from the surface of the
Infection- relationship between the host and the parasite, and the ensuing struggle
for supremacy (if host loses = disease)
Normal Flora- infection that does not cause disease
1. Prevents bacterial cells from rupturing when water pressure inside is greater than
2. Responsible for shape of cell
3. Serves as point of anchorage for flagella
Teichoic acid- Gram positive cell, 25nm peptidoglycan ?
70 points 7 = 10 points
Laboratory Report Exercise Six (SS 2013)
Name_ Lab Section_
TA_ Place the tape with your unknown # here: 1pt
Data: Your results for your unknown
1 04 points total 8 = 13 points total
L aboratory Report Exercise Four ( SS 2013)
T A_1pt_ Lab Section_1pt_
S treak Plates
T TC Stabs
drawing: 2 points
color: 2 points
organism: 6 points
Total: 10 points x 2 = 20 points