Chapter 6 problems:
1. A population of molecules in which it is easy to study evolutionary processes using
All of the above.
None of the above.
2. Why are protein comparisons of three-dimensional
Study Problems Ch. 3
1. Proteins can be separated from small molecules and ions through a semi-permeable membrane
2. In the Edman procedure for peptide sequence, phenyl isothiocyanate is used to selectively
remove the _ residue as a PTH-derivative.
Chapter 2 Problems
Choose the correct answer from the list below. Not all of the answers will be used.
a) L-amino acids
e) secondary structure
f) tertiary structure
Chapter 1 Problems
1. The structure of DNA described by Watson and Crick included
a double helix.
the sugar phosphate backbone aligned in the center of the helix.
the base pairs that are stacked on the inside of the double helix.
a and b.
Carbonic Anhydrase makes Fast
Remember, CO2 released into
blood streamreacts with what to
Reaction proceeds on order of
Zn is nec. for activitycarbonic
anhydrase metalloprotein NOT
Zn bound to imidaz
Principles Use Basic Catalytic Strategies
General Acid-Base Catalysis
Catalysis by Approximation
Metal Ion Catalysis
Proteases Facilitate Hard Reactions
Examining the Specificity of
May also react at large
What is Biochemistry?
The chemistry of lifes processes.
What are biochemical molecules?
Macromolecules- Proteins, Nucleic Acids
Low MW- glucose, glycerol, amino acids
Molecule responsible for storing of
There is a link between
Many factors can influence Km
pH, temperature, ionic
Km essentially has 2
1)[S] where of the active sites
on the E are occupied.
2)Related to the rates of
individual steps (k1, k-1, k2)
Turnover Number and Catalytic
Enzymes Powerful and Highly
Accelerate reactionsdoes not mean
that they force unreactive species to
reactSee the difference?
Can be highly specific for the
reactions that are catalyzed involving
reactants (aka substrates)
Catalytic activity ca
A Portrait of a Protein in
Looking at Hb/Mb shows glaring
similarities and glaring differences
as it reltes to protein structure and
nearly identical structures for O2
Function of O2 binding efficiency
Members of similar molecular
detail can have similar features
Can be detected by comparing
3-D structuremost linked to
properties such as function
Sequence comparison is a
powerful tool in the analysis of
How Genomics and Proteomics are complimentary
Recombinant DNA technology has allowed for the
acquisition of much of amino acid sequence information
The Structural Biology of Antibodies
immunoglobulin)is a protein.
Synthesized due to the
The first step involving the study of proteins for the
purpose of understanding structure-function relationships is
purifying the protein of interest
Must be seperated from other proteins in the cell based on:
Solubility, size, charge
Peptide Sequences Can Adopt Different
Conformations in Different Proteins?
Due to tertiary interactions with residues in other
parts of the protein that are brought into close
proximity to sequences and facilitate different
interactions in surrounding e
2 Structure: Chains can Fold into Regular Structures
Structural regions help dene overall topology of protein
-helix, -sheet, -turns, loops
Different views of the -helix
Hydrogen Bonding in -helix
The CO group of the ith residue H-bonds with the
Protein Composition and Structure
Several key properties of proteins
Linear polymers built of monomer units of amino acids
Contain wide range of functional groups
Interact with each other or other bio macromolecules to form complexes