Page 1 Bio 3324 Molecular Biology Exam II November 19, 2009
This exam is worth a total of 100 points 1. a. The abundance of various proteins varies enormously within a cell. Give three levels of regulation that may account for this variance. (3 pts.) b. G
page 1 Bio 3324 Molecular Biology Exam I October 13, 2009
This exam is worth a total of 100 points 1. a. Give two methods that can be used to denature double stranded DNA. (2 pts.) b. What bonds are being broken and are they covalent or non-covalent? (2 p
Solving a restriction map The solution of a circular plasmid restriction map will include all restriction enzyme cutting sites for the given enzymes as well as the total length of the plasmid in kb and the distance in kb between each cut. 1. Only maps tha
Practice RFLP problem a) The gene for sickle cell anemia is inherited with the RFLP XL322 94.7% of the time. It is inherited with the RFLP NM502 98.6% of the time. Both of these RFLPs are used diagnostically to predict who will inherit the gene for the di
Practice Restriction Map Given the following results of restriction digests, draw the circular restriction map of the plasmid. Fragment legnths are given in kb (10 pts.) RI 9.8 0.8 RI/BglII 6.2 3.6 0.8 BglII 6.2 4.4 HindIII 10.6 HindIII/RI 8.3 1.5 0.8 Hin
Take-home Final
Bio 325/807
Spring 2006
Take-home final exam that is due in my office by noon on Friday April 28, 2006. You may hand it in earlier if you wish. You are expected to consult your laboratory manual, the reference book Molecular Cloning by J.
Chapter 12 Negative regulator - a repressor protein binds to an operator to prevent a gene from being expressed Positive regulator - a transcription factor is required to bind at the promoter in order to enable RNA polymerase to initiate transcripti
page 1 Bio 324 Molecular Biology Exam II November 14, 2006
1. a. In transcription, one of the two DNA strands is copied into RNA. Is the same DNA strand used to sythesize all RNAs in the cell, or are different RNAs copied from different DNA strands? (3 pt
Bio 324
Molecular Biology
Exam I
October 10. 2006
This exam is worth a total of 100 points 1. a. The basic building block of nucleic acids is the nucleotide. It is composed of three components, what are they? (3 pts.) phosphates, nitrogenous base (purine
