TNS Exam 2 Review:
Electrophysiology
Electrical properties of neurons
o Voltage separation across the membrane
Sodium potassium pump and maintenance of separation of charge
Separation of charge and how it can be used to do work
Direction of flow of io
TNSexam2
Measuringtherestingmembranepotentialofaneuron
Typicallyaseparationofchargebetweeninandoutsideofcell
o Utilizethatseparationofchargetosendsignals
Stickinganelectrodeintoornexttoacellreadingthevoltageinthecell
o Movementsofchargesorionsfromintoou
TNS Exam 2
Fluorescence microscopy
Two important wavelengths
- will determine filter to be used
- absorbed and released
Filters used to block unwanted wavelengths
- fluorophores (colored fluorescent proteins)
- colocalization
Excitation filter - specific
TNS exam 2
Measuring the resting membrane potential of a neuron
- Typically a separation of charge between in and outside of cell
o Utilize that separation of charge to send signals
- Sticking an electrode into or next to a cell reading the voltage in the
