Courtney Docherty Seat 12 April 16, 2009 Induction of -Galactoside Objective: In this experiment we are measuring the activity of the lacoperon by the use -Galactosidase, which will cleave ONPG. When the ONPG is cleaved it will produce glucose and ON
Quiz Question Pool 6 Spring 2017
Hint: you may have to consult your textbook (Chapter 7.4 for metabolic pathways) for some of the
Questions on metabolic pathways:
1) What are the nutritional needs of wildtype Neurospora (excluding water)? (3 poin
Quiz Question Pool 4 Spring 2017
ALWAYS SHOW YOUR WORK. Be sure your symbols for dominant & recessive alleles are
1. In cross I, we are treating F1 x dp se (cross [b] on page 34) and dp se x F1 (cross [c] on page 34)
Quiz Question Pool 5 Spring 2017
Hint: see your textbook Chapter 7.3 for info on phage recombination; Chapter 9 contains info on
cloning. NB: the answers to most questions can be found in your lab manual!
Questions on phage:
1) What is a cistron? (2 point
Quiz Question Pool 1 Spring 2017
Below you will find a list of possible questions for the first of the weekly lab quizzes, which will be
administered the week beginning January 23; your instructor will choose 10 points worth of
questions from the list. Th
Quiz Question Pool 2 Spring 2017
ALWAYS SHOW YOUR WORK! Avoid the use of ambiguous symbols for dominant vs.
1) Why will you remove the parents from the cross vials on week #2 of a fly cross? (2 points)
2) Draw a corn kernel and label th
23 pairs: 46 total chromosomes
Variable differences in size and shape between chromosome pairs
Within pairs appear to match exactly ( X and Y are exceptions)
Chromosome theory of inheritance chromosomes are the cellular structures responsible fo
Chapter 15 - How Genes Are Regulated; Gene Regulation in Prokaryotes
15.1 The Elements of Prokaryotic Gene Expression
15.2 Regulation of Transcription Initiation via DNA-Binding Proteins
15.3 RNA-Mediated Mechanisms of Gene Regulation
15.4 Discovering and
1. Chapter 16 - How Genes Are Regulated; Gene Regulation in Eukaryotes
16.1 Overview of Eukaryotic Gene Regulation
16.2 Control of Transcription Initiation Through Enhancers
16.3 Epigenetics: Control of Transcription Initiation Through DNA Methylation
Chapter 11 - The Eukaryotic Chromosome
Chromosomes have a versatile, dynamic structure that supports their many functions in the
packaging, replication, segregation, and expression of the information in a single long molecule
11.1 Chromosomal DNA a
Problem 2.16. What is the probability of producing a child that will nmgggicall;
resemblemof the two parents in the following four crosses?
a.Aa Bb Cc Dd X aa bb cc dd
b.Aa bb cc ddXAABB CC DD
c Aa Bb Cc: Dd X Aa 1313 CC Dd ' -_
d.aa bbcc dana bbcc dd W
3.10. Several genes in humans in addition to the ABO gene (I) give rise to recognizable
antigens on the surface of red blood cells. The MN and Rh genes are two examples.
The Rh locus can contain either a positive or a negative allele, with positive being
Molecular Pharmacology Fast Forward. Published on February 24, 2011 as doi:10.1124/mol.111.071290
Cannabidiol and other cannabinoids reduce microglial activation in vitro and in vivo:
relevance to Alzheimers disease
Ana Mara Martn-Moreno
1. In cross I, we are treating F1 x dp se (cross [b] on page 34) and dp se x F1 (cross [c] on
page 34) as equivalent crosses, but in Drosophila that is generally a very bad idea! Why is it a
bad idea in general and why will we be able to get away with it
F1 x F1 cross
P: se x dp
F1: se+se/dp+dp (wild type)
F2: (resulting from a F1 x F1 cross)
total:55 total:14 total:16 total:3
Genes: basic unit of biological information; specific segment of DNA in a discrete region of a chromosome that
serves as a unit of function by encoding a particular RNA or protein.
Heredity: the way genes transmit physiological, physical, and behavioral t
Homozygous: a genotype in which the two copies of the gene that determine a particular trait are the same allele.
Heterozygous: a genotype in which the two copies of the gene that determine a particular trait are different alleles;
Cross I Segregation, Independent Assortment
Objective: to describe if the Drosophila melanogaster traits dp (dumpy) and se (sepia) follow
Mendels Laws of Inheritance.
The F1 generation all appear to be wild-type (red eyes and oval wings).
F1 x F1
Epistasis: A gene interaction in which the effects of an allele at one gene hide the effects of alleles at another gene
Complementary gene action: requires 2 enzymes to catalyze two separate biochemical reactions to change a trait.
Ex. 2 White flowers pur
sister chromatids: the two identical copies of a chromosome that exist immediately after DNA replication. Sister
chromatids are held together by protein complexes called cohesins.
centromere: a specialized chromosome region at which sister chromatids are
3.34. The garden ower Salpigiossis sinuata (painted tongue") comes in many
different colors. Several crosses are made between true-breedin arental
strains to produce F1 plants, which are in turn self-fertilized to produce F2
Parents F1 henot as F
Chapter 12 - How Genes Travel on Chromosomes; Chromosomal Rearrangements and
Changes in Chromosomes number
Chromosome rearrangement - major feature of evolution
Syntenic segments - identity, order, and transcriptional direction of genes are almost