Lab Practical Part 1
Highlights the unique growth patterns of microbes on an O2 gradient
(facultative anaerobe, strict aerobe, strict anaerobe, and microaerophiles.
1) colony 2) heat fix and air dry 3) methyl blue 4) rinse
Exam 2 Section 2
an organism that cannot grow in the presence of oxygen
organism that REQUIRES A CONSTANT SUPPLY OF OXYGEN in order
an organism that prefers to live in a non-oxygen environment b
-Members of this group serve as vectors, transmitting several different viruses, bacteria, and protozoan
parasites between hosts
- Members of this group have hard exoskeletons and jointed appendages
-Members of this group come in fl
Hydrogen Sulfide test Lab page 167-68
1. Distinguish between the types of substrates available to cells for H2S production
Organic sulfur which is a component of peptones. If can break the amino acid cysteine, it can
produce H2S. Inorganic sulfur is in th
Hydrogen Sulfide Test p. 167
Color of medium
IMViC Test p. 161
SIM Agar Deep
If Red layer idole positive
Part B Methyl Red (if red + result)
Microbiology Student Guidelines
Mechanisms of Antimicrobial Action
What is selective toxicity?
Why are there more antibacterial rugs than antifungal, antiprotozoal,
antihelminthic or antiviral drugs?
Identify the 6 mechanisms by
STUDENT GUIDELINES CHAPTER 9
Basic Principles of Microbial Control
Define the following:
What is microbi
Microbiology: Student Guidelines
How can microbes be classified?
1. a. What is a taxonomic system?
b. How did Linnaeus group organisms?
c. How have todays biologists modified Linnaeuss system?
d. List 5 groups of microorganisms.
e. Although micr
9 biggest threats to college
Why do nurses
have to take
Urease Test page 171
1. Mechanisms of urease activity?
Urease breaks the bonds between carbon and nitrogen to form the end product of ammonia, which
makes the environment around it become alkaline
2. Function of phenol red in the urea broth medium?
IMViC Test page 163
1. Discuss the medical significance of the IMViC series of tests?
will determine the biochemical properties and enzymatic reactions of enterobacteriaeceae. The IMViC
will determine whether they are pathogenic, occasional pathogenic, or
Exam 2 Section 1
How does smear preparation of cells from a liquid media differ from
preparation of cells from a solid medium?
you get less bacteria from liquid and you don't need to start with a drop of
Why is it important to limit the quanitity o
Exam 2 Section 3
Why does exposure to UV radiation cause death in vegetative cells but
UV does no cause death to endospores because they have proteins around
the DNA that provide protection from the damaging affects from UV.
At which wave
Lab 1 Part 2
For preparation of a smear on a slide, what is the purpose of heat
fixation? What problems can arise when the slide is heated in the flame
Heat fixation of smears kills the bacterial cells and causes them to adhere to
the glass so that they d
Lab Practical Part 2
Phenol Red Test
VP-color to red
bubble formation by adding hydrogen peroxide
clearing around growth
color change to pink
Milk media. clearing
Chapter 11 Notes, part 2
An aerobic heterotroph are thermophilic and are heat resistant. Used as DNA
polymerase in polymerase chain reaction.
An aerobic heterotroph that serves as a facultative anaerobe in the intestinal
Chapter 11 Notes, part 1
studied photosynthetic purple bacteria and noted similarity of photosynthetic
reactions in all photosynthetic organisms.
Bacteria that are able to make their own food, usually by fixating a gas such
as CO2 or N
At what temperature does agar solidify?
40-45C. Quiz 1
Where do glass slides go in lab after use?
envelope, far end of the bench. Quiz 1
What is a means of growing anaerobic and microaerophilic bacteria?
anaerobic jar LAB 4
A positive chromagen is
Ch 15 HW answers
Virulence Factors: Endotoxins:
Endotoxins are also known as
When would endotoxins be released from a bacterial cell?
When the cell dies
Which of the following would be the first sign of an infection that resulted in the release of
Ch 17 HW answers
Host Defenses: The Big Picture
Mucous membranes are a part of
According to the animation, B cells interact directly with
helper T cells.
Which of the following defense systems would be involved in eliminating virally-infec
Part A Q6: Which of the following characteristics do commensalism, mutualism, and pathogenicity have
-life cycle coordination between the host and symbiont
-common physical requirements between the organisms
Part A: Which of the following
Ch 16 HW answers
Q45 Which non-specific defense mechanism is mismatched with its associated body structure or body
mucociliary escalator intestines
Q4 Which group contains ONLY innate physical defenses
intact skin, tears, ciliary escalator
Its a co
Chapter 13 Notes
1) nucleic acid (DNA or RNA)
2) protein coat
Two essential components of a virus are:
The protein coat of a virus is called.
Viruses that infect only prokaryotes a and are usually double stranded DNA
with a capsid an