NMR - Recall From Last Week
From 1H NMR, we get:
Chemical shift data () - This tells us what kinds of protons we have.
Integration data - This tells us the ratio of each kind of proton in our sample.
1H - 1H coupling data - This tells us about protons
Chapter 8 Biocalorimetry
Lenore M. Martin PhD
URI-BCH521-Physical
Biochemistry
Biocalorimetry allows direct measurement of enthalpy changes
associated with chemical reactions and processes.
Two main types of calorimetry are in widespread use
nowadays:
Iso
Magnetic Resonance Imaging
Theory
MRI is an application of nuclear
magnetic resonance spectroscopy
(NMR)
Some types of nuclei create magnetic
fields
Most MRI techniques use 1H nuclei
Properties unique to magnetic nuclei
are:
1) Non-zero angular moment
Optical Rotatory Dispersion (ORD), Circular Dichroism (CD) units and conversions
Specific Rotation: []T = 100 /cl
(rot angle/path): = (1800/) x (nL-n R)
when c is in g/100mL and l is in dm, T in C is the wavelength of incident light.
Reduced residue rotat
NMR STUDIES OF STRUCTURE AND FUNCTION
OF BIOLOGICAL MACROMOLECULES
Nobel Lecture, December 8, 2002
by
KURT WTHRICH
Eidgenssische Technische Hochschule Zrich, CH-8093 Zrich, Switzerland, and The Scripps Research Institute, 10550 N. Torrey Pines Rd., La Jol
BCH521 Physical Biochemistry - Paper and Presentation Guidelines
Fall 2010 Prof. LM Martin Cell and Molecular Biology
As scientific professionals, you will be expected to explore new biochemical techniques on your
own, and then respond to questions about
NMR = Nuclear Magnetic Resonance
Physical Principles:
Some (but not all) nuclei, such as 1H, 13C, 19F, 31P have nuclear spin.
A spinning charge creates a magnetic moment, so these nuclei can be thought of
as tiny magnets.
If we place these nuclei in a mag
Chapter 3-Spectroscopic
Techniques: Part 3
NMR, ESR, X-ray
Prof Lenore M. Martin PhD
URI-BCH521-Physical Biochemistry
Department of Cell & Molecular Biology
Physical Basis of NMR
spectrometry
Figure 3.48: A spinning
nucleus generates a
magnetic field with
WhatisMassSpectrometry?
Mass spectrometry is an analytical technique used to identify
compounds by measuring the mass-to-charge ratio of ions. It is
most generally used to find the composition of a physical sample
by generating a mass spectrum representin
URI
BCH521
Physical Biochemistry
Prof Martin
Fall 2010
General Term Definitions Used in Discrete Mathematics
Discrete Mathematics: The branch of mathematics dealing with objects that can assume
only distinct, separated values.
Algorithm: A specific set of
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