There are 33 questions, 3 points each except question 1 is 4 points. Don't submit till you finish the last question or you are out of time.
1. Following statements, which one is INCORRECT about protein production?
The rate of t ranscription regulates p
There are 33 questions with only one correct answer for each question (3 points each). You have 60 min to finish the exam. Don't submit till you have finished the exam. 1. Which of the following statement about eukaryotes and prokaryotes is INCORRECT? A.
What procedure is used to identify a protein in a gel?
Why would you want to attach an epitope tag to a protein you are studying?
An epitope is a portion of a molecule to which an antibody binds. Epitopes can be
Chapter 12 Study Guide (Lectures 33, 34 and 35): Intracellular Compartments /
1. List 6 organelles found in a eukaryotic cell? Which of these organelles is not
found in animal cells?
2. Would proteins in the lumen of the ER have disulfide
2015 NITTANY NOTES ALL RIGHTS RESERVED
ANY ATTEMPT TO REPRODUCE THESE NOTES IS
Check out nittanynotes.com for Online notes! | 814-238-0623
Like us at Facebook.com/NittanyNotes
Exam Pack #2
1 OF 38
PUNISHABLE BY LAW
Textbook Reading: 263-285, 287-294.
Why is DNA replication said to be semiconservative?
DNA replication is said to be semiconservative because when a Parent DNA is
replicated, one strand in each of the DNA is from the parent DNA.
To which carbon o
What is the end replication problem and how do eukaryotes deal with it?
The end replication problem arises because the leading strand can be synthesized the
entire way to the end of the strand but the lagging strand cannot because it needs an
Three different types of RNA are involved in translation. What are they and what are
their functions? Which RNA contains codons and which RNA contains anticodons?
mRNA This is the RNA responsible for protein synthesis. Contains codons.
Why is DNA replication said to be semiconservative?
To which carbon of the deoxyribose in the incoming nucleotide is linked to which carbon
of the existing chain being elongated by DNA polymerase? Which direction does the
What is the n
1.) During the cotranslational transport process, start transfer and stop transfer
sequences control the incorporation of transmembrane domains into the ER
membrane. Only one of these two sequences interacts with SRP and the protein
Lecture 30 (Reese)
Ion Channels and the electrical
properties of membranes.
The phospholipid bilayer is a barrier that controls the transport of
molecules in and out of the cell.
Charged molecules are virtually
impermeable. Involves transporters and
Lecture 18 :Gene regulatory
Post-transcriptional control of
Genetic versus epigenetic regulation of expression
Epigenetic: inheritable changes in gene expression that does not
require changes in DNA sequ
Membrane proteins: Since The lipid bilayer is impermeable,
movement of materials and communication between the
inside and outside of the cell are mediated by membrane
As with most aspects of a cell, protein
Membrane protein and small
Membrane proteins are often classified as peripheral membrane proteins or
integral membrane proteins based on what is required to extract the protein
from a membrane.
Integral membrane proteins - th
post-translational protein modification
and lipid biosynthesis
Nascent polypeptides enter the ER lumen unfolded
Chaperone called BiP in the lumen associates with hydrophobic surfaces to prevent
Transporters and active membrane
The phospholipid bilayer is a barrier that controls the
transport of molecules in and out of the cell.
Remember: the lipid bilayer
is impermeable to many essential
molecules used by the cell
Primary Goal: Get an internship at Samsung.
Dream Job: Become a cyber security officer for Samsung.
Define Success: Gain experience through internships.
Define Success: Travel the world with my friends.
Peroxisomes have a single membrane
They perform oxidation reactions that breakdown fatty acids and toxic molecules
that enter the cell from the blood stream.
ER > Golgi
When the protein is properly folded, the protein is collected in COPII
coated vesicles. These vesicles fuse with each other to form the
vesicular tubular cluster that then fuses with the cis-Golgi network.
Control of proteins (cont.)
Regulation by Phosphorylation
KINASES add phosphates to proteins
-phosphate is derived from ATP
-side chains with -OH groups can be
phosphorylated (serine, threonine,
Chromatin and DNA synthesis
Human genome: 3,000,000,000 bp X 0.34 nm/bp X 2 copies =
How does the 3 meter genome fit in a 5 micrometer diameter
DNA in the nucleus is packaged with proteins in a complex
called chromatin. When
Components of a protein
20 amino acids
Polypeptide = protein
Figure 3-1 Molecular Biology of the Cell ( Garland Science 2008)
Steric collisions restrict the path of the polypeptide ba
Control of Gene Expression: DNA
Formation of different cell types occurs by synthesizing
different RNA and proteins, not by permanent changes in
the DNA sequence.
The formation of a specific type of cell is
Gene regulatory mechanisms I
Prokaryotic switches II
Eukaryotic transcription and
The Lac operon was
one of the first
Lac operon is
two factors, a
BMB/Micro 251 Molecular & Cellular Biology I
Dr. Joseph Reese, 463A North Frear, 865-1976, jcr8@
Dr. Lui Bai, 206B LSB [email protected]
Class notes and other materials are posted on ANGEL.
Office hours in 463A North Frear: TBD or by appointment.
Chapter 2. Cell Chemistry and Biosynthesis.
Cells are made of relatively few elements: C, O, N, and H are
Rarer but essential elements include P, S, Cl, Ca, K, Na, Fe.
(Includes water which
constitutes 70% of the
Vesicular formation and targeting
Once proteins that dont normally reside in the ER are
properly folded, they are transported to the Golgi apparatus
Figure 13-3 Molecular Biology of the Cell ( Garland Science 2008)
Hereditary info, encodes for
characteristics, transfers to
large coding capacity
base = phosphate
Is easy to break
because it is made up
KNOW ALL OF THE BULLSHIT
DNA Damage and Repair
3 steps to high-fidelity DNA synthesis
1. 5-3 polymerization
2. 3-5 exonucleolytic proofreading
3. strand-directed mismatch repair
The 4 Rs of repair
Most likely d
BMB 251 Exam 4
Intracellular Compartments and Protein Sorting
Intracellular Membrane Traffic
Transport of molecules between nucleus and
Transport thru nuclear
PRODUCTION PROCESS ENGINEERING
Hydrate Formation and Prevention
Dr. Hamid Emami-Meybodi
PNG 480 Spring 2017 Hydrate Formation and Prevention
is a concept used to describe the phenomena of
precipitation & deposition of s
Become familiar with the following
phenomena and their impacts on weather:
El Nino / Southern Oscillation (ENSO)
Madden Jullian Oscillation (MJO)
Chapter 9 part 2 Learning Objectives
State the enzymes involved in lipid hydrolysis and the products of the hydrolysis reactions.
State what the term lyso refers to in reference to lipids.
State basic structure and roles of DAG and LPA.
Recognize the name