Review for 454 Standard Final:
My SUGGESTIONS for Stryer Chapter reviews.
I will give you what my guess are the key chapters and concepts to study. This does
not mean they are the ONLY concepts to study.
Chapter 2, 3 and 4. Pay close attention to biochemi
Purines (2 rings:pyrimidine and imidazole) - Adenine, Guanine are purines derivatives in nucleic acid
Pyrimidines (one ring) - Cytosine, Thymine, and Uracil (in RNA only) are pyrimidine derivatives in nucleic acid
Z DNA - 12 bp per turn. No groove
1/Vo = (Km/Vmax)(1/[S]) + (1/Vmax)
Km is an indicator of substrate binding affinity.
Low Km = tight binding
Michaelis-Menten kinetics parameters:
1. Initial Rate = V0 (i.e. no product no K-2)
2. Formation of ES quickly reaches equilibriu