A restriction enzyme is an enzyme that cuts double-stranded DNA at specific sites known as Restriction
recognition sequences. PUC19 is an artificially plasmid created by university of California, its a versatile
tool for genetic manipulation.
Plasmid Isolation and Quantification
A plasmid made out of small, circular, double stranded DNA molecule that is separated from cell
chromosomal DNA. Plasmids are common in prokaryotes and some eukaryotes. In most cases plasmid
Agrose gel electrophoresis is the process that we used to identify and analyses DNA and RNA strands and
its done by separating the genetic material by its size. The genetic material is placed in the wells of the