CHEM 527 Problem set 1 (due: Sep. 26) 1. An organic compound (A) is composed of carbon, hydrogen and nitrogen, with carbon constituting over 60% of the mass. It shows a molecular ion at m/z=112 amu in the mass spectrum. Answer the following two questions:
An Introduction to Mass Spectrometry CONTENTS
1. What is mass spectrometry (MS)? What Information does mass spectrometry provide? 2. Where are mass spectrometers used? 3. How can mass spectrometry help biochemists? 4. How does a mass spectrometer work? 1.
CHEM 527: Chemical and Biochemical Characterization Methods
Instructor: Dr. Bukuo Ni Office: STC 303 Phone: 903-886-5382, e-mail: [email protected]rce.edu
Required Textbook: Spectrometric Identification of Organic Compounds, 7th Edition, Robert M. Silve
Covalent and Non-Covalent Bond Energies
C-H, C-C bonds ~300-400 kJ/mol Non-covalent bonds 10-100 x weaker * their weakness allows them to be continually broken and reformed in a dynamic molecular interplay that is life.
Types of Non-Covalent Interactions
capacitytopolymerize novelacidbaseproperties variedstructureandchemicalfunctionality chirality
-carbon is chiral (except for glycine) at pH 7.0 unchar
O O O H3N CH C N CH C N CH C O CH2 H CH3 HH CH2 S MetAlaGly CH3
1. Theorderofaminoacidsina proteinisknownastheproteins sequence=primarystructure.
Conformationspatialarrangementofatomsina protein Nativeconformationconformationoffunctional protein
Simple composed only of amino acid residues Conjugated contain prosth
Chapte 4: Part 2 r
Prote 3-D structure 3 and 4 structureand in : prote folding. in
3o S tructure
ve in third le l of prote organization
folding of polype ptidechain cause 2 structure to inte s s ract ino que nd the rm Am acids that arefar apart in s
Chapter 4 (part 3)
How does the structure of a protein affect its biological function?
Animal Scrapie:s h e e p TME(tra ns m is s ib le m inke nc e p h a lo p a th y) :m ink CWD(c h ro nic wa s ting d is e a s e ) :m ule d e e r,e lk BSE(b o vine s p o ng
The quantitative study of how organisms gain and use energy If you want to understand bioenergetics, you have to understand thermodynamics.so lets review some thermodynamics System: Could be a bacterial cell, a petri dish of bacteria cells,