How does agarose gel electrophoresis work to separate different size fragments of
Through agarose gel electrophoresis DNA fragments separate based on their size and
charge. The supercoiled fragments move faster due to less friction. Next is linear
Describe the size and structure of pLAF including the function of the various
components. Compare the natural function from how components of the lac operon
such as the lac promoter and the lac-Z gene are utilized in biotechnology and
(a.) The mini-prep DNA procedure you performed is referred
to as Alkaline SDS Lysis DNA
prep. Explain what causes the cells to lyse during the procedure. What reagent
establishes the high pH? How does the alkalinity affect plasmid DNA? How does it
DNA by itself is not visible to the naked eye when it is in solution. How can one
visualize DNA fragments in a gel? To visualize DNA in this exercise, what
precautions must be taken?
The dye EZ vision 2 stains DNA which is then excited by ultraviolet ligh
Using the linear regression equation, estimate the approximate size of each of the
DNA band(s) found in each of the samples.
PL RD (-): x = 0.5 cm; hence Y= 3.9356. Hence, approximate size of PL RD (-) DNA is
PL RD (+): x= 1 cm and 0.8 cm. Henc
Proper pH is essential to ALL biological processes. Discuss how changes in pH affect
inter-molecular and intra-molecular interactions (H-bonds, van der Waals interactions,
ionic bonds, hydrophobic interactions, etc.) of biological molecules.
A student, who has not learned how to properly prepare solutions, added X g of Y solid
material (FW =?) to exactly 150.0 mL of dH2O and assumed that a 0.5 M solution had been
prepared. The total solution volume increased to Z mL after the solution was tho
Using the entire class data set, explicitly compare the three devices and discuss what might
account for any differences by answering the following:
Which of the three devices was the most accurate for measuring a 60- mL volume?
Include the statistic
GROUP: Analyze the data obtained for the three devices used to measure a 60-mL volume.
Determine each of the three statistics (mean, standard deviation, and PEM) for the
three devices using the provided data template for both your group data set
Define the term pH. For the unknown solution given in Section 1.9, what was the initial
pH and H+ concentration (include units as appropriate)? Was this unknown solution
initially more acidic or more basic than distilled deionized water?
pH is a scal
A buffer resist the change in pH of the solution even if we add acid or base within its buffer
range. We have used 2M HCl as an independent variable since we manipulated its quantity. In
this case pH of the solution is dependent variable which varies due