BIO 205L: Laboratory Experiments in Cell & Molecular Biology
Lecture 1: Introduction
Structure and Function of Organisms
Who is Who?
Lectures 1 - 4 & Course Website Lectures 5 - 8 Lectures 9 -12
Dr. Moon Draper Instructor
Dr. Bill Allen
REVIEW QUESTIONS Labs 2 and 3 Types of Microscopy For each specimen listed below, describe which technique (brightfield, darkfield, phase contrast, fluorescent microscopy) you would use to visualize it. 1) An unstained, live amoeba
2) Genetic material in
REVIEW QUESTIONS Lab 4 Population Growth Kinetics What do we mean by positive growth kinetics?
A pure culture grown under optimal conditions exhibits four distinct phases of growth. Name each phase and describe why it occurs.
The mathematical equation for
REVIEW QUESTIONS Lab 5 Development Describe the main stages of sea urchin development
Describe the main stages of corn seedling development.
Except in rare cases, no more than one sperm is able to fertilize an egg. Describe the cellular mechanisms that pr
REVIEW QUESTIONS Lab 6 Separation Science True or False: 1) The higher the Rf of a band on a silica TLC plate, the more polar the molecule in that band 2) Using a DEAE-cellulose column with Tris buffer as the eluant, a more negatively charged particle wil
REVIEW QUESTIONS Labs 7, 8, and 9 Protein Extraction When proteins are to be extracted from whole cells, several experimental protocols must be followed to preserve protein structure insofar as possible. Please explain the purpose of each of the following
REVIEW QUESTIONS Labs 10, 11, and 12 PCR The parameters for a PCR you are running are listed below. Explain the significance of each temperature, and why PCR is a chain reaction. 94 C 94 C 55 C 72 C 4 C
You have a DNA sample containing 5000 copies of a se
Exam covers (Lots of stuff)
Making solutions Microscopy Population kinetics Chromatography Urchin and root embryonic development Protein Extraction Protein electrophoresis & Western Blotting Plasmid Ligation and Bacterial Transformation Isolation
BIO 205L Review
Western Blotting Isolation of Plasmid DNA Plasmid Ligation and Bacterial Transformation Ligation and Bacterial Transformation Analysis of Restriction Digestion of Plasmid DNA Polymerase Chain Reaction Protein & DNA
REVIEW QUESTIONS Lab 1 Measuring Devices Which of the following devices is appropriate to measure the stated quantity? 1. 23.4 g NaCl a) analytical balance b) two-pan balance c) top-loading balance 2. 5.4 ml water a) blow-out pipette b) graduated cylinder
List for these couple of weeks
Get ready for final Complete evaluations for LORs and LIs Provide feedback on both teaching and course improvements
SELECTION AND ANALYSES OF TRANSFORMED BACTERIA AGAROSE GEL ELECTROPHORESIS ANALYSES OF RESTRICTI
Things to Learn From This Week's Lab
Polymerase Chain Reaction (PCR) GENETIC ENGINEERING OR RECOMBINANT DNA TECHNOLOGY
Polymerase Chain Reaction to Amplify Large Quantities of DNA in a Few Hours How to use restriction enzymes to cut DNA in speci
Lecture 2 (Week3): Brightfield, Darkfield &! Phase Contrast Microscopy" BIO 206L! ! Fluorescence Microscopy
BIO 205L !
Structure and Function of Organisms
What We Can Do with Microscopy
Microscopy allows us to examine very small structures
To be Learned in Exercise 4!
! Observation and measurement of growth kinetics! ! Be able to identify the parts of corn seedling.! ! Be able to identify stages of mitosis in prepared slides of corn seedling roots.! ! How to initiate and recognize
Metabolic Reactions: Enzymatic Activity and Environmental Effects Photosynthesis: Chloroplast Isolation and Function Cell Respiration: Mitochondrial Structure & Function
! A. William Allen! ! PAI 1.22G!
Group Analysis 5 !
! You may complete the group Analysis in the way that is easiest for you. ! ! With your normal Tuesday lab group, your new group if thing
Extraction and Analysis of Proteins From Cells
Things to Learn in This Week's Lab
1. How to extract proteins from cells.
2.! How to make parallel dilutions from a stock solution. 4.! How to determine protein concentration using a standard curve
Electrophoresis and Electroblotting of Proteins
! Monday evening ! October 25 7:30-9:00 PM ! Exercises 1-7 ! 8 AM Lecture meet in ART 1.102 ! 1 PM Lecture meet in UTC 2.112A
7:309:00 PM on Monday, October 25th 2010 8 AM Lecture the exam is in ART 1.102 (map) 1 PM Lecture the exam is in UTC 2.112A (map) 3 PM Lecture the exam is in BUR 106 (map)
You MUST take the exam in the room as designated according to the Lect
DNA Bioinformatics Extraction of Plasmid DNA Extraction of Genomic DNA
What You Will Learn from This Class-DNA Bioinformatics The Alkaline Lysis Mini-Prep Procedure to Mini Prep Isolate Plasmid DNA from Bacterial Cells Quick Extraction of Genomi
The following is a list of some procedures/concepts which to be familiar for the Bio205L practical exam. Note: it is NOT all-inclusive. Lab safety rules knowledge and demonstration of good laboratory practices Use of safety equipment / supplies gloves, go