CHE 339/BME 339/BIO 335
Exam I
Thursday, October 2, 2007
Your name _
Part 1: Short Questions: ANSWER IN THE SPACE PROVIDED
1. Name 4 ways for measuring cell growth (4 points).
1)
2)
3)
4)
Direct counting (microcope count, particle counting, single cell fo
Fall 2010 BME339/CHE339/BIO335
HW#4
Solutions
Problem 1
Known:
F = 1000 mL /hr = 1 L/hr
YMX/S = Y = 0.5 g cells/ g gluc
So = 10 g gluc / L
m = 0.2 hr-1
C = 1.5
V = 1000 mL = 1 L
(Max yield; no maintenance)
Monod Kinetics/Subs. Lim. growth
KS = 1 g gluc/L
CHE 339/ BME 339/BIO 335
Fall 2010
Homework #2
Assigned: Sept. 7, 2010
Due: Tuesday, September 14, 2010
1. You are working as a biotechnologist at Archie Daniels Midland on a process development
team. Your team is designing a process to extract all possib
CHE 339/ BIO 335/BME 339
Fall 2010
Homework #4
Assigned: Sept. 21, 2010
Due: Tuesday Sept 28, 2010
1. In a chemostat with cell recycle, the feed flow rate and culture volumes are F = 100 ml/h
and V = 1000 ml, respectively. The system is operated under glu
FINAL EXAM 2009
BME 339/CHE 339/BIO 335
SECTION 1: Short Answer questions
1. Bioreactors and animal cells (8 points)
A. Explain using the appropriate questions how you would carry out an experiment to determine
Lecture 9 CHE/BME339: Take a break: Review Problem Solving
What is the purpose of a phase I clinical trial?
Lecture 8: safety with healthy people
Name three advantages of recycle continuous stirred tank reactors?
Name 4 ways for measuring cell growth:
Lec
BME 339/CHE 339/BIO 335
Fall 2006
Homework #1
Assigned: Sept. 7 2006
Due: Thursday, September 14 2006
1) The respiratory quotient for the bacterium Rhodococcus erythropolis was experimentally
determined to be equal to 1.4 gr CO2 produced per gr of O2 cons
1)
a. Calculation of the Steady State Biomass:
The reactor is continuous and has no recycle stream The Reactor Balance on S
yields:
(
)
This equation will allow us to solve for the steady state biomass given:
Thus the steady state biomass is:
(
)
(
)
Calc
1) Problem 1
a. Both endogenous metabolism and death rate are negligible.
The Reactor Balance on X yields:
=
0 = 0
=
=
The Reactor Balance on S yields:
=
( ) =
(1)
= ( )
Given Equation:
+
=
=
(2)
Now by combining equations (1) and (2) an
1.
The enzyme NcoI recognizes the sequence
5' -C^C A T G G-3
3' -G G T A C^C-5'
It is used to cut the genome of a bacterium whose genome consists of 65% G or C and
35% A or T. The genome of the bacterium is 2,800,000 bp. How many fragments would you
estim
BME 339/CHE 339/BIO 335
Fall 2014
Homework #3
Assigned: Tuesday September 30th, 2014
Due: Tuesday, October 7th, 2014
1) In a chemostat with cell recycle, the feed flow rate and culture volumes are F = 100 mL/hr
and V = 1000 mL, respectively. The system is
2. An aerobic batch fermentation is conducted at 37 C. Previous experiments have shown that the
parameter kLa is constant throughout the fermentation and equal to 2000 h-1. The doubling time of the
cells in exponential growth is 50 min, the oxygen yield c
BME 339/CHE 339/BIO 335
Fall 2014
Homework #2
Assigned: Thursday September 11th, 2014
Due: Thursday, September 18th, 2014
1) You are operating a chemostat (that doesnt contain a recycle loop) in which the cell culture
does not obey the Monod model, but gr
BME 339/CHE 339/BIO 335
Fall 2014
Homework #4
Assigned: Tuesday October 7th, 2014
Due: Tuesday, October 14th, 2014
1) Pseudomonas putida with umax = 0.5 hr-1 is cultivated in a continuous culture under aerobic
conditions where D = 0.28 hr-1. The carbon an
1)
p(G) = p(C) = p(A) = p(T) = 0.25 = 1/4 (for parts a c)
a.
(
)
( ) ( ) ( ) ( ) ( ) ( ) (
)
XbaI will cut once every 4096 basepairs given a G-C content of 50%
b.
(
)
( ) ( ) ( ) ( ) ( ) ( ) ( )
(
) (
BspMI will cut once every 4096 basepairs given a G-C c
BME 339/CHE 339/BIO 335
Fall 2014
Homework #5
Assigned: Tuesday October 14th, 2014
Due: Tuesday, October 21st, 2014
1) Assuming that an organisms genome has a G-C content of 50%, with what frequency would
you expect the following restriction enzymes to cu
BME 339/CHE 339/BIO 335
Fall 2014
Homework #1
Assigned: Thursday September 4th, 2014
Due: Thursday, September 11th, 2014
1) A yeast (CH1.66N0.13O0.40) is growing aerobically on arabinose (C5H10O5) and ammonium
hydroxide (NH4OH) with a respiratory quotient
SECOND EXAM
CHE 339/BIO 335/BME 339
FALL 2005
7:00-8:30 pm
Thursday, November 3, 2005
Regrades are possible only if your responses are in ink.
YOUR NAME _
1. List and briefly describe the steps in making a cDNA library. An antibody against the protein
enc
FINAL EXAM
BME 339/CHE 339/BIO 335
FALL 2006
Saturday, December 16, 2006
(Total 103 points including 3 bonus points)
YOUR NAME _
1. A. Derive expressions for the biomass concentration, X, and the substrate concentration, S,
in the outflow of a continuous
FINAL EXAM
CHE 339/BIO 335/BME 339
FALL 2003
9:00-12:00 NOON
Wednesday, December 10, 2003
1) The gene fraternity is thought to be responsible for a disorder characterized by complete
lapse of mental function in young adult males, particularly following mo
BME 339/CHE 339/BIO 335
Fall 2005
Homework #1
Assigned: Sept. 8, 2002
Due: Thursday, September 15, 2002
1) The respiratory quotient for the bacterium Rhodococcus erythropolis was experimentally
determined to be equal to 1.4 gr CO2 produced per gr of O2 co
BME 339/CHE 339/BIO 335
Fall 2006
Homework #1
Assigned: Sept. 7 2006
Due: Thursday, September 14 2006
1) The respiratory quotient for the bacterium Rhodococcus erythropolis was experimentally
determined to be equal to 1.4 gr CO2 produced per gr of O2 cons
CHE 339/ BME 339/BIO 335
Fall 2006
Homework #2
Assigned: Sept. 19, 2006
Due: Thursday, September 26, 2006
1. Exam problem 2004 In a chemostat with cell recycle, the feed flow rate and culture
volumes are F = 100 ml/h and V = 1000 ml, respectively. The sys
BME 339/CHE 339/BIO 335
Fall 2014
Examples Problems
1) In the production of bacterial toxins the growth rate depends on the concentration of the toxin
product P and the concentration of the limiting substrate S. In some cases (for low dilution
rates), the