Automated DNA Sequencing
The DNA, if double-stranded is denatured
A primer complementary to a DNA
fragment is hybridized to the single
A polymerase amplifies the
Syllabus: BIOE 111 (Spring 2014)
Course Number: BioE 111
Course Title: Functional Biomaterials Development and Characterization
Instructor: Seung-Wuk Lee, Assistant Professor of Department of Bioengineering
1-220 Donner Lab
Department of Bioengineering,
In this chapter, you will learn about . . .
o . . . the biomolecules that are most commonly analysed in bioanalytical
chemistry: amino acids, proteins and nucleic acids.
o . . . the structure of these biomolecules and their physical
Professor In-Geol Choi
College of Life Sciences &
Why do we sequence DNA?
How do we sequence DNA?
DNA technology electrophoresis & PCR
Traditional sequencing methods
How do we sequence Ge