Huxford-Chem 560-Fall 2015
Department of Chemistry & Biochemistry
Office: CSL 325 (inside of the lab)
Phone: (619) 594-1597 (Lab)
(619) 594-1606 (Office)
Three Dimensional Structure of
Distinguish primary, secondary, tertiary, and quaternary structure.
Describe and draw the peptide group and explain how polypeptides fold up using
terms like and angle.
Recognize and describe
Column contains a specic ligand!
Mixture of proteins runs through the column!
Proteins with afnity for the ligand stay behind!
Elution: free ligand, change of pH, salt concentation!
Elution prole of an
Nucleic Acid Structure!
Nucleic acids are polymers of nucleotides!
DNA is a polymer of deoxynucleotides!
RNA is a polymer of ribonucleotides !
Nucleotides are linked by 3-5 phosphodiester bonds
Sequences are written 5 3
Watson and Cric
Prebiotic soup contains building blocks!
Association with membranes!
Selection for systems that copy themselves faster!
Problems need to be solved!
after association with membranes!
Standard state conventions
Temperature = 25C (298K)"
Pressure = 1 atm"
activity of solutes = 1"
activity of pure water is assigned 1 "
H+ activity at pH 7.0 = 1"
Life obeys the laws of thermodynamics
Living organisms are open syste
An acid can donate a proton"
A base can absorb a proton"
HA + H2O"
H3O+ + A-"
H3 O+ ][ A ]
K a = K [H 2 O ] =
H 3O + ][A ]
pKa = logK a
Strength of an acid refers to its"
' Chem 560
Fall Semester, 2015
Quiz 225 points possible
Answer the questions that follow. Points available for each problem are given in
1) (8 points) The figure on the right shows a ribbon diagram representation of t