There are multiple ways of quantifying the protein concentrations of cellular
homogenates and other subcellular functions. These methods include the Lowry, Bradford,
Bicinchoninic acid method (BCA), and Biuret assay. The BCA and Biuret assay
Lab Report # 2
Even if the compound microscope is familiar to you, there are some features that may
take you by surprise, such as how to optimize the alignment of the lens system to enhance the
Cuvette water and dye. Cuvette and water have the capacity to absorb light. Omit both of
these by setting the cuvette and water to zero.
Interpretation of the data (consider the following questions):
1. Does the mixture of two light absorbing dyes also
EDTA preservative. Preserve protein. Inhibit protein degradation by Why are we keeping solutions on
ice? lowers the activation energy, lowering the kinetic activity, and keep bacteria from eating proteins.
Tris used to maintain pH.
KCl Salt, solubilize pr