Problem Set 6
The following table indicates the rate at which a substrate reacts as catalyzed by
an enzyme that follows Michaelis-Menten mechanism: 1) in the absence of inhibitor; 2)
and 3) in the presence of 10 mM concentration, respectively, of each
Problem Set 2
Draw the general structure of an amino acid in both the L and D configurations. Which configuration
would be found in nature?
Give the name, three-letter symbol, and one letter symbol of any nonpolar amino acid that c
Chem 365 Problem Set 3
Ovalbumin is first, then fibrinogen, hemoglobin, and finally lysozyme.
b) anode +
cathode D AC EB
a) A is first, then B, and finally C
b) A is first, and t
Problem set 3
In what order will the following proteins be eluted from a CM-cellulose ion exchange resin (cation
exchange column) as the pH of the elution buffer is ramped from a pH of 3 to a pH of 12:fibrinogen
(pI=5.8), hemoglobin (pI=7.1,
Problem Set 1
Chapter 1 and 2
Draw the hydrogen bond(s) that are likely to form between the following pairs of molecules:
a) methanol and water
b) two molecules of ethanol
Calculate the pH of a) 0.1 M HCl b) 0.1 M NaOH c) 3x10-5 M HNO3 d) 5x
Problem Set 1
Note: See bulletin board for complete answers showing all calculations.
See bulletin board for structures.
See bulletin board for titration curve.
a) 3.7 b) unprotonated
a) 5.4 b) 8.8
Oct. 13, 2004
Do all of the questions.
Part I. All questions are worth 5 points each.
Multiple Choice: Circle the single, best answer
All of the following statements are true about the relationships between [S], Km and Vmax EXCEPT:
Do all of the following 21 questions.
Multiple Choice: Circle the single, best answer.
The lipid shown on the right represents what class of lipids?
e) none of the a
Nov. 29, 2004
Answer all of the following questions.
Part I. Multiple Choice. Circle the single, best answer.
Glucokinase has a Km value of 10.0 mM, whereas hexokinase has a Km value of 0.1 mM
that is consistent with:
Problem Set 4 Answers
DTT cleaves disulfide bonds. Since insulin has 2 disulfide that hold the A and B chains together, DTT
would cleave insulin into its A and B chains. These chains separately would no longer be able to
perform insulins prope
Problem Set 4
Refer to figure 5.1 in book for problem 1.
If DTT (dithiothreitol) were added to a basic buffered solution of insulin, what effect would this have on
Name three ways in which a protein could be denatured in
Problem set 7
The lipid portion of a typical bilayer is about 3 nm thick.
a) Calculate the number of residues in an helix that will just span the distance. The pitch of an
helix is 0.54 nm/turn.
b) The epidermal growth factor receptor has a
a) no effect b) increases c) increases d) increases
O2 +2H+ + 2e- H2O
NADH + H+ NAD+ + 2 H+ + 2e- oxidation
Overall reaction: O2 + NADH + H+ H2 O + NAD+
Oxygen is the oxidizing agent, and NADH is the reducing agent.
The NADH and FADH2
Problem Set 8
Chapters 16 and 17
What is the effect of increasing the concentration of each of the following on the rate of the TCA cycle?
a) Coenzyme A, (b) acetyl-CoA, (c) NAD+, (d) vitamin B
The following half reactions play important roles in me
Problem Set 7 Answers
a) This is simply a matter of using dimensional analysis:
3 nm * 1 turn
* 3.6 residues = 20 residues
The underlined portion are the amino acids the will form the single transmem
Problem Set 5 Answer Key
Covalent cross-linking contributes significantly to the strength and rigidity of collagen fibers. In one
type of cross- link, allysine residues condense with lysine residues to form a Schiff base. When an
Problem Set 5
Homocysteine is an -amino acid containing one more methylene group in its side chain.
Homocysteinuria is a genetic disease characterized by elevated levels of homocysteine in plasma and
urine, as well as skeletal deformities due to defect